TIA-1通过影响肝细胞转录因子的活性调节HBV的e抗原和s抗原表达

陈婧; 李毅; 谭畅; 刘湘; 黄爱龙; 汤华

您当前的位置：

首页 >

文章列表页 >

TIA-1通过影响肝细胞转录因子的活性调节HBV的e抗原和s抗原表达

论著 | 更新时间：2026-01-09

- TIA-1通过影响肝细胞转录因子的活性调节HBV的e抗原和s抗原表达
- TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions
- 陆军军医大学学报 2008年30卷第9期页码：834-836
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R373.21;R392.11;R392.2
- 纸质出版：2008
- 稿件说明：
移动端阅览
陈婧,李毅,谭畅,刘湘,黄爱龙,汤华.TIA-1通过影响肝细胞转录因子的活性调节HBV的e抗原和s抗原表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(09):834-836.

　CHEN Jing,LI Yi,TAN Chang,LIU Xiang,HUANG Ai-long,TANG Hua.TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(09):834-836.
陈婧,李毅,谭畅,刘湘,黄爱龙,汤华.TIA-1通过影响肝细胞转录因子的活性调节HBV的e抗原和s抗原表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(09):834-836. DOI：

　CHEN Jing,LI Yi,TAN Chang,LIU Xiang,HUANG Ai-long,TANG Hua.TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(09):834-836. DOI：

摘要

目的证明TIA-1分子具有调节HBV基因表达的功能。方法 TIA-1真核表达质粒pcDNA3-TIA-1，用脂质体转染HepG 2.2.15细胞后，ELISA检测e和s抗原的表达；RT-PCR检测与HBV复制有关的转录因子HNF1、 HNF2、 HNF3、HNF4、 RXRα、 PPARα、C/EBP的表达。结果与正常的HepG 2.2.15细胞相比，转染pcDNA3-TIA-1质粒的HepG 2.2.15细胞，其e抗原的表达量明显的增高，而s抗原表达量明显的降低。与正常的HepG 2.2.15细胞相比，转染pcDNA3-TIA-1质粒的HepG 2.2.15细胞中的HNF1、 HNF2、 RXRα mRNA含量有明显的下降。结论 TIA-1蛋白能通过影响与HBV复制有关的转录因子活性，调节HBV e抗原和s抗原的表达。

Abstract

Objective To identify TIA-1 function in regulating HBV replication. Methods pcDNA3-TIA-1 eukaryotic expression vector was transfected into HepG 2.2.15 hepatoma cell line with lipofectamine. HBV e antigen and s antigen was checked with ELISA method. RT-PCR was used to identify expression of some transcription factors that involved in HBV transcription such as HNF1

HNF2

HNF3

HNF4

RXRα

PPARα

and C/EBP. Results Compared with normal HepG 2.2.15 hepatoma cell

the expression of e antigen was obviously high and s antigen was depressed in the HepG 2.2.15 hepatoma cell transfected with pcDNA3-TIA-1 vector. Compared with normal HepG 2.2.15 hepatoma cell

the mRNA level of HNF1

HNF2

RXRα changed significantly. Conclusion These data show that TIA-1 may interact with liver-enriched transcription factors and regulate HBV e antigen and s antigen expression.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

转录因子SP1上调STMN1促进子宫平滑肌瘤细胞增殖并抑制凋亡

转录因子LMO3对子宫内膜癌患者预后的影响

胃黏膜肠化的H3K27me3沉默子重塑特征及转录调控机制

胃黏膜肠上皮化生的H3K27ac增强子重编程特征及转录调控机制

MAPK信号通路对人胆管癌细胞Fas配体基因表达的调节及其机制的初步探讨