PARP抑制剂5-AIQ对CT26细胞侵袭及转移的影响

黎明; 蔡莉; 王娅兰

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PARP抑制剂5-AIQ对CT26细胞侵袭及转移的影响

论著 | 更新时间：2026-01-09

- PARP抑制剂5-AIQ对CT26细胞侵袭及转移的影响
- Effect of poly(ADP-ribose) polymerase inhibitor 5-AIQ on invasion and metastasis of CT26 cells
- 陆军军医大学学报 2008年30卷第3期页码：237-240
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  中图分类号： R73-362;R73-37;R735.35
- 纸质出版：2008
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黎明,蔡莉,王娅兰.PARP抑制剂5-AIQ对CT26细胞侵袭及转移的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):237-240.

　LI Ming,CAI Li,WANG Ya-lan.Effect of poly(ADP-ribose) polymerase inhibitor 5-AIQ on invasion and metastasis of CT26 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(03):237-240.
黎明,蔡莉,王娅兰.PARP抑制剂5-AIQ对CT26细胞侵袭及转移的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):237-240. DOI：

　LI Ming,CAI Li,WANG Ya-lan.Effect of poly(ADP-ribose) polymerase inhibitor 5-AIQ on invasion and metastasis of CT26 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(03):237-240. DOI：

摘要

目的探讨多聚（腺苷二磷酸核糖）聚合酶[Poly(ADP-ribose) polymerases

PARPs]抑制剂5-氨基异喹啉酮[5-Aminoisoquinolinone·HCl

5-AIQ]对小鼠结肠腺癌CT26细胞基质黏附、运动、侵袭能力的影响。方法应用Western blot检测5-AIQ对PARP表达的影响，细胞基质黏附实验、细胞运动及侵袭实验观察5AIQ抑制CT26细胞PARP后，其基质黏附、运动及侵袭能力的变化。结果 5-AIQ处理组PARP表达明显弱于未处理组（P0.05）；黏附实验结果显示，与5-AIQ未处理组比较，5-AIQ 100、500 μmol/L处理组的吸光度值明显降低（P0.01），其黏附抑制率分别是14.54%、21.69%；运动及侵袭实验中，5-AIQ 500 μmol/L处理组与未处理组的细胞数差别显著（P0.05），其运动及侵袭抑制率分别是30.09%、37.47%。结论 5-AIQ可抑制CT26细胞PARP的表达，并可降低CT26细胞基质黏附、运动及侵袭能力，5-AIQ 在抗肿瘤侵袭转移中可能发挥作用。

Abstract

Objective To study the effect of poly(ADP-ribose) polymerase (PARP) inhibitor 5-AIQ on the adhesion

migration and invasion of mouse colon adenocarcinoma cell line (CT26). Methods The expression of PARP after 5-AIQ treatment was detected Western blot. The cell adhesion

migration and invasion of CT26 after 5-AIQ treatment were observed by cell-matrix adhesion assay

cell migration assay and matrigel invasion assay. Results The expression of PARP in 5-AIQ-treated CT26 was weaker than that in 5-AIQ-untreated cells (P0.05). In the adhesion assay

the OD value of 5-AIQ-treated cells was reduced as compared with untreated cells (P0.05) and the inhibition rate of 100 μmol/L 5-AIQ treated cells and 500 μmol/L 5-AIQ treated cells was 14.54% and 21.69% respectively. Moreover

migration and invasion potential of CT26 were also reduced by 500 μmol/L 5-AIQ treatment (P0.05)

and the inhibition rate was 30.09% and 37.47% for migration and invasion respectively. Conclusion PARP inhibitor 5-AIQ can reduce the expression of PARP

adhesion

migration and invasion of CT26 cells

suggesting 5-AIQ probably plays a role against tumor metastasis.

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