Objective To explore the proliferation of endothelial progenitor cells (EPCs) after the transfection of human VEGF165

and investigate the re-endothelialization of the transfected cells in artery intimal injury rats.  Methods After isolated from rat bone marrow

EPCs were cultured and then identified by FITC-UEA-I and Dil-acLDL. EPCs cells were transfected with pcDNA3-hVEGF165 or pcDNA3 by DOTAP Liposome. After transfection

the expression of VEGF was detected by using ELISA．The EPCs proliferation was studied by MTT methods. VEGF-transfected EPCs were transplanted into experimental artery intimal injury rats and its effect on injured vascular re-endothelial was studied.  Results Cells expressed double FITC-UEA-I and Dil-acLDL fluorescence were confirmed to EPCs. The medium of EPCs transfected with pcDNA3-hVEGF165 had expression of VEGF. VEGF165 gene transfer stimulated EPCs adhesion

migration and proliferation. Gene-modified EPCs transplanted into rats

survived in intimal vascular injury site

repaired intimal vascular injury better than those in normal EPCs group.  Conclusion VEGF165 gene is successfully transfected into EPCs

which can stimulate EPCs’ migration

adherence and proliferation. VEGF-transfected EPCs have stronger intimal repair ability compared with non-transfected EPCs. VEGF-transfected EPCs repair injured vascular intima

restore normal vascular endothelial structure.