Objective To isolate a bio-effective fraction from a traditional Chinese medicine cape jasmine fruit and evaluate its anti-LPS bioactivity.  Methods The fraction

named D and targeting on lipid A (the bioactive component of LPS)

was isolated from the water extracts of cape jasmine fruit by using the techniques

such as macroporous adsorptive resins

solvent extraction

polyamide laminar analysis under the screen of an affinity biosensor. Purer components CJ-1 were obtained after high performance liquid chromatography (HPLC). The neutralizing activity to LPS (0.2 μg/L) of CJ-1 at different concentrations (0.5

1.0

2.0 mg/L) were further examined by kinetics turbid metric limulus. Enzyme linked immunosorbent assay (ELISA) was also used to detect CJ-1’s effect on LPS-induced release of tumor necrosis factor-α (TNF-α) from RAW 264.7 cells.  Results The isolated components CJ-1 have high binding ability to lipid A (RU value: 112 arc/s). CJ-1 (0.5

1.0

2.0 mg/L) inhibit 0.2 μg/L LPS-induced coagulation of limulus reagents significantly and 100 μg/L LPS-induced TNF-α secretion both in a dose-dependent manner (P0.05).  Conclusion Biosensor technique is effective in guiding the isolation of CJ-1 from water extracts of cape jasmine fruit. CJ-1 has high affinity to lipid A and is able to neutralize LPS in vitro.