Objective〖WTBZ〗To determine the mutations of exons 5

6

7 and 8 of p53 gene and the alteration of microsatellite DNA around it

and investigate their relationship in T-cell lymphoma.  Methods We measured frequencies of p53 gene point mutation in exons 5 to 8 by PCR-SSCP methods and detected microsatellite alteration (MA) including microsatellite instability (MSI) and loss of heterozygosity (LOH) by PCR-based methods at 4 microsatellite loci (D17S945

D17S938

D17S947 and D17S926) around p53 gene in tumor mass and adjacent normal lymphatic tissue or reactive hyperplasia lymph node excised from 38 cases of T-cell lymphoma.  Results The frequency of p53 gene mutation

MA

and LOH was 39.47%(15/38)

23.68%(9/38)

and 7.89%(3/38)

respectively. At the 4 microsatellite loci

the frequency of MSI and LOH varied from 2.86% to18.42% and from 0 to 5.26%

respectively. The mutation frequency of p53 gene in MA positive cases was 55.56%(5/9)

and in MA negative group was 34.48%(10/29)

but the difference was not significant(P 0.05). Additionally

at D17S926 and D17S947

the mutation frequency of p53 gene in LOH positive cases was 100% and in the negative cases was 36.11%(P0.05).  Conclusion There are MA and mutation of p53 gene in T-cell lymphoma though no significant correlation between them. But

MA positive cases might experience high mutation of p53 gene in T-cell lymphoma.