Objective To study the effect of powder of Chinese Angelica and Peony (DSS) on hippocampus proteome of senescence accelerated mice (SAM).  Methods Thirty female mice aged 12 months were used in our study

including 10 SAM-resistance/1 (SAMR1) and 20 SAMpprone/8 (SAMP8). The difference of hippocampus proteome among SAMP8

SAMR1

SAMP8 treated with DSS intragastrically at dose of 3.2 g/kg once a day for 30 d (SAMP8+DSS)

was analyzed by two dimensional polyacryalmide gel electrophoresis (2DE).  Results Compared with SAMR1

the expressions of 13 proteins in hippocampus of SAMP8 were up-regulated and that of 9 proteins were down-regulated significantly. Compared with SAMP8

the expressions of 21 proteins in hippocampus of SAMP8+DSS were up-regulated and that of 14 proteins were down-regulated significantly. Using MALDI-TOF-MS

the proteins with significant changes in expressions were identified by peptide fingerprinting map and the results were searched in MASCOT database

and sorted into 5 function groups including mitochondria-associated proteins

energy metabolism proteins

synaptic plasticity formation proteins

cytoskeletal proteins and others proteins.  Conclusion The effects of DSS on hippocampus proteome of senescence accelerated mice were probably exerted via  multi-target and multi-pathway mechanism. 