Objective To investigate the effect of human telomerase reverse transcriptase (hTERT) gene on normal human dermal papilla cells.   Methods The hTERT gene was introduced into cultured normal human dermal papilla cells by Liposome-mediated transfection

then the positive cells were selected with G418. After the positive cells were passaged on

the integration and expression of hTERT gene were detected and the biological characteristics of the transfected cells were analyzed.   Results A positive cell clone was selected

and the 35th passage of transfected dermal papilla cells was obtained so far. Expression of hTERT gene was detected on DNA

mRNA and protein levels. The transfected cells possess the biological characteristics of normal human dermal papilla cells.   Conclusion The lifespan of normal human dermal papilla cells can be extended by hTERT gene transfection

and the transfected cells keep the normal biological characteristics.