培养神经元细胞中E3B1基因的表达变化与轴突生长抑制的关系

向强; 周跃; 李长青; 郑文杰; 吕宏琳

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培养神经元细胞中E3B1基因的表达变化与轴突生长抑制的关系

论著 | 更新时间：2026-01-09

- 培养神经元细胞中E3B1基因的表达变化与轴突生长抑制的关系
- Relation between E3B1 gene expression in cultured cortical neurons and axon growth
- 陆军军医大学学报 2007年29卷第24期页码：2301-2303
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  中图分类号： R322.81;R329.2;R394.2
- 纸质出版：2007
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向强,周跃,李长青,郑文杰,吕宏琳.培养神经元细胞中E3B1基因的表达变化与轴突生长抑制的关系[J].陆军军医大学学报(原第三军医大学学报),2007,29(24):2301-2303.

　XIANG Qiang,ZHOU Yue,LI Chang-qing,ZHENG Wen-jie,LU Hong-lin.Relation between E3B1 gene expression in cultured cortical neurons and axon growth[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(24):2301-2303.
向强,周跃,李长青,郑文杰,吕宏琳.培养神经元细胞中E3B1基因的表达变化与轴突生长抑制的关系[J].陆军军医大学学报(原第三军医大学学报),2007,29(24):2301-2303. DOI：

　XIANG Qiang,ZHOU Yue,LI Chang-qing,ZHENG Wen-jie,LU Hong-lin.Relation between E3B1 gene expression in cultured cortical neurons and axon growth[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(24):2301-2303. DOI：

摘要

目的检测E3B1基因在培养神经元轴突生长抑制前后的表达变化及意义。方法应用RT-PCR和Western blot检测培养神经元轴突生长抑制前后E3B1 mRNA和蛋白的表达，应用免疫细胞化学检测培养神经元Ⅲ型β-微管蛋白的表达。结果与正常培养的神经元相比，轴突抑制物共培养的神经元细胞Ⅲ型β微管蛋白表达明显降低(P0.01)，E3B1 mRNA和蛋白的表达均显著升高(P0.01)。结论 E3B1基因的表达变化与神经元细胞轴突的生长抑制有密切关系。

Abstract

Objective To investigate the expression of E3B1 gene in cultured neurons and its significance in axon growth. Methods The mRNA levels of E3B1 were measured by RT-PCR and the protein level by Western blot analysis. The expression of type Ⅲ β-microtubulin of E3B1 was detected by immunocytochemistry. Results Compared with those in the normal cultured neurons

the expression of type Ⅲ β-microtubulin was lower in the neurons with axonal growth restricted (P0.01)

and their mRNA and protein levels of E3B1 were obviously higher (P0.01). Conclusion The expression changes of E3B1 gene has a close relation with neurite outgrowth.

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