SHI Yan,HAO Ying-xue,LEI Xiao,YU Pei-wu,QIAN Feng,TANG Bo,ZHAO Yong-liang,RAO Yun.Effect of mimic CO2 pneumoperitoneum on cell proliferation and cell cycle of human gastric cancer MKN-45 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2274-2276.
SHI Yan,HAO Ying-xue,LEI Xiao,YU Pei-wu,QIAN Feng,TANG Bo,ZHAO Yong-liang,RAO Yun.Effect of mimic CO2 pneumoperitoneum on cell proliferation and cell cycle of human gastric cancer MKN-45 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2274-2276.DOI:
Objective To study the effects of mimic carbon dioxide pneumoperitoneum on cell proliferation of human gastric cancer cells. Methods The gastric cancer cells MKN-45 were exposed to CO
2
environment for 4 h under the pressure of 12 mmHg and those cultured in normal experiment condition as control. The cell proliferation of MKN-45 cells were examined by MTT method on day 1-7 after exposure and their cell cycle by flow cytometry 24 h after exposure. Results The cell proliferation and cell proliferative index (PI) showed no statistical difference between CO
2
pneumoperitoneum group and control group (P0.05). Conclusion The ce
ll proliferation and cell cycle of human gastric cancer MKN-45 are not significantly affected after exposure to CO