Objective    To explore the proliferative and apoptotic effect of 3 different siRNAs targeting survivin in human bladder cancer cell line EJ28.     Methods    Three siRNAs targeting survivin and 1 fluorescence-labeled siRNA as a negative control were chemically synthesized. Transfetion efficiency was observed under fluorescence microscope after transfecting the fluorescence-labeled siRNAs. EJ28 cells were divided into 6 groups: siRNA164 group

siRNA167 group

siRNA389 group

and negative control group

lipofectin group and cell control group. The proliferation of EJ28 cell was detected by MTT assay at 24

48 and 72 h after the transfection. Apoptotic rate was detected by Annexin V-FITC-FCM assay at 48 h．    Results    All the sequence-specific siRNAs targeting survivin efficiently suppressed the proliferation of EJ28 cells. siRNA 164 had the most powerful effect because of its highest inhibitory rate of (41.32±2.54)% and the highest apoptotic rate of (13.20±0.25)% at 48 h after transfection.     Conclusion    RNAi targeting survivin has a potential value in the gene therapy of bladder cancer.