硫芥对中国仓鼠肺成纤维细胞DNA的损伤及还原型谷胱甘肽的保护作用

李奇慧; 董兆君; 孙华斌; 黄尉初; 杨新芳; 王修德; 唐木涛; 赛燕

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硫芥对中国仓鼠肺成纤维细胞DNA的损伤及还原型谷胱甘肽的保护作用

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- 硫芥对中国仓鼠肺成纤维细胞DNA的损伤及还原型谷胱甘肽的保护作用
- Reduced glutathione protects against DNA damage of CHL cells by sulfur mustard
- 陆军军医大学学报 2007年29卷第23期页码：2250-2253
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  中图分类号： R394.2;R977.4;R996.1
- 纸质出版：2007
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李奇慧,董兆君,孙华斌,黄尉初,杨新芳,王修德,唐木涛,赛燕.硫芥对中国仓鼠肺成纤维细胞DNA的损伤及还原型谷胱甘肽的保护作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(23):2250-2253.

　LI Qi-hui,DONG Zhao-jun,SUN Hua-bin,HUANG Wei-chu,YANG Xin-fang,WANG Xiu-de,TANG Mu-tao,SAI Yan.Reduced glutathione protects against DNA damage of CHL cells by sulfur mustard[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2250-2253.
李奇慧,董兆君,孙华斌,黄尉初,杨新芳,王修德,唐木涛,赛燕.硫芥对中国仓鼠肺成纤维细胞DNA的损伤及还原型谷胱甘肽的保护作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(23):2250-2253. DOI：

　LI Qi-hui,DONG Zhao-jun,SUN Hua-bin,HUANG Wei-chu,YANG Xin-fang,WANG Xiu-de,TANG Mu-tao,SAI Yan.Reduced glutathione protects against DNA damage of CHL cells by sulfur mustard[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2250-2253. DOI：

摘要

目的研究硫芥对中国仓鼠肺成纤维细胞（Chinese hamster fibroblast cell line

CHL）DNA的损伤作用及还原型谷胱甘肽（reduced glutathione

GSH）的保护作用。方法将CHL分为2组，均以10、 100、 1 000 μmol/L硫芥染毒，其中1组以10 mmol/L的GSH加以保护，分别在不同时间点（即刻

6、24、48、72 h）收集细胞进行单细胞凝胶电泳（single cell gel electrophoresis assay

SCG）检测。结果硫芥染毒组CHL的DNA迁移率和迁移度在染毒后6、24、48、72 h均显著高于相应时刻的生理盐水组和溶剂对照组 (P0.01)。GSH保护组DNA迁移率和迁移度亦显著增高 (P0.01)，但较之单纯硫芥染毒组DNA迁移率下降了13.0%~52.5%，迁移度亦显著下降。结论硫芥对CHL的DNA有损伤作用，呈时间、剂量关系。GSH对DNA的损伤有一定保护作用。

Abstract

Objective To study the DNA damage of Chinese hamster fibroblast cell line (CHL) by sulfur mustard and the protective effect of reduced glutathione (GSH). Methods CHL cells were divided into two groups and harvested to assay by single cell gel electrophoresis at 0

72 h after exposure to 10

100

1 000 μmol/L sulfur mustard respectively. In one group

10 mmol/L GSH was added to study its protective effect. Results The rate of DNA migration and the length of DNA migration of CHL cells exposed to sulfur mustard at 6

72 h were significantly higher than those treated with normal saline or solvent (P0.01). In GSH group

those increased but were still less than those in sulfur mustard group

and the rate of DNA migration decreased by 13.0%-52.5%. Conclusion Sulfur mustard could induce DNA damage of CHL cells and the damage displayed in time and dose dependent manners. GSH could partly protect cells against DNA damage induced by sulfur mustard.

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