小鼠整合素α<sub>4</sub>基因克隆、序列分析及突变修复

王永明; 胡燕; 黎海芪

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小鼠整合素α₄基因克隆、序列分析及突变修复

论著 | 更新时间：2026-01-09

- 小鼠整合素α₄基因克隆、序列分析及突变修复
- Molecular cloning, sequence analysis of mouse integrin α₄ gene and repairing of its mutation
- 陆军军医大学学报 2007年29卷第22期页码：2131-2134
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  中图分类号： R322.45;R394-33;R394.3
- 纸质出版：2007
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王永明,胡燕,黎海芪.小鼠整合素α4基因克隆、序列分析及突变修复[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2131-2134.

　WANG Yong-ming,HU Yan,LI Hai-qi.Molecular cloning, sequence analysis of mouse integrin α4 gene and repairing of its mutation[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2131-2134.
王永明,胡燕,黎海芪.小鼠整合素α4基因克隆、序列分析及突变修复[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2131-2134. DOI：

　WANG Yong-ming,HU Yan,LI Hai-qi.Molecular cloning, sequence analysis of mouse integrin α4 gene and repairing of its mutation[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2131-2134. DOI：

摘要

目的克隆小鼠整合素α

基因cDNA，进行分析整合素α

基因序列，对造成氨基酸突变的碱基进行定点突变修复。方法从小鼠小肠peyer’s结获得α

基因cDNA，采用RT-PCR方法，克隆到pMD18-T载体，选择阳性克隆进行酶切鉴定和序列测定，对造成氨基酸突变的碱基进行定点突变修复。结果扩增得到的α

基因cDNA为3 099 bp，编码1 032 个氨基酸，与GenBank中公布的序列比较，有12个碱基发生突变，其中6个碱基造成6个氨基酸发生改变，对发生突变的6个碱基进行定点突变修复。结论获得小鼠整合素α

基因的克隆。

Abstract

Objective To clone and analyze the full-length cDNA of mouse integrin α

and repair the mutation sensible locei that caused the change of amino acids. Methods The cDNA of α

gene was amplified by RT-PCR using the total RNA extracted from mouse small intestine peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed into E. coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of α

cDNA that caused the change of amino acids was repaired. Results The cDNA of mouse α

had a length of 3 099 bp

and encoded a product of 1 032 amino acids. There were 12 bases pairs mutati

on of α4 gene and the 6 base pairs causing the change of amino acids was repaired. Conclusion The cDNA of mouse α

is cloned successfully.

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小鼠整合素α4基因克隆、序列分析及突变修复

Molecular cloning, sequence analysis of mouse integrin α4 gene and repairing of its mutation

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摘要

Abstract

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Keywords

references

小鼠整合素α₄基因克隆、序列分析及突变修复

Molecular cloning, sequence analysis of mouse integrin α₄ gene and repairing of its mutation