YANG Xian,YANG Shui-ping,ZHANG Xue,TAN Jie.Optimization of taxol extraction from Taxus baccata L.[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(19):1886-1888.
YANG Xian,YANG Shui-ping,ZHANG Xue,TAN Jie.Optimization of taxol extraction from Taxus baccata L.[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(19):1886-1888.DOI:
Objective To optimize and establish the extractive technology of taxol from Taxus baccatal L.. Methods HPLC was applied to determine the content of taxol. The orthogonal test was adopted to examine the effects of the 4 factors consisting of ethanol concentration
ethanol volume
extractive times and extractive time. Results Ethanol concentration and ethanol volume were essential factors influencing the extraction of taxol. High concentration and amount of ethanol facilitated the extraction. Extractive times and extractive time were also important. By single-factorial effect
2 h and 2 times in each extraction of taxol were the best. Conclusion The optimum extractive condition is T9 (A