WU Xiao-juan,YAO Zhen-wei,TANG Jun-ying,YAO Chen-guo,SUN Cai-xin.Growth inhibition and apoptosis induction of nanosecond steep pulse on human ovarian carcinoma cell line SKOV3[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(17):1670-1672.
WU Xiao-juan,YAO Zhen-wei,TANG Jun-ying,YAO Chen-guo,SUN Cai-xin.Growth inhibition and apoptosis induction of nanosecond steep pulse on human ovarian carcinoma cell line SKOV3[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(17):1670-1672.DOI:
Objective To investigate the effect of nanosecond steep pulse on growth and apoptosis of human ovarian carcinoma cells in vitro. Methods MTT assay
invert microscopy,fluorescence microscopy and annexinV-FITC were used to identify the inhibitory effect of nanosecond steep pulse on cell proliferation and the induction of apoptosis in human ovarian carcinoma cell Line SKOV
3
. Results Nanosecond steep pulse with 10 and 12.5 kV/cm significantly inhibited the viability of SKOV
3
cells in a dose-dependent manner
and in a time-dependent manner within 2 to 4 h of nanosecond steep pulse treatment. The typical changes of SKOV3 cell apoptosis were observed by i
nvert microscopy and fluorescence microscopy. Early apoptotic rate of SKOV
3
treated with 10
12.5 kV/cm nanosecond steep pulse was(22.21±2.71)% and (57.30±6.51)%
respectively
significantly higher than those of the control group(3.04±0.44)% (P0.05). Conclusion Nanosecond steep pulse in certain range can inhibit the growth of SKOV