Objective    To construct a RhoC-siRNA expression vector

and study its role on the biological behaviors of hepatoma carcinoma cells.　     Methods    RhoC-siRNA gene was synthesized and cloned into the expression vector pSilencer2.1. The constructed RhoC-siRNA expression vector was stably transfected into hepatoma carcinoma cell line SK-Hep1. The inhibitory effect of RhoC-siRNA on the expression of RhoC in transfected cells was detected by Western blotting. The morphous

growth velocity and the ability of cell adhesion

cell migration

cell invasion before and after transfection was observed.     Results    Enzyme digestion and DNA sequencing confirmed that the RhoC specific siRNA expression vector was constructed successfully. After transfection

RhoC expression was inhibited by 60%

and no marked difference was observed in cellular morphous and growth curve

while the ability of cell adhesion

cell migration

and cell invasion were markedly decreased.     Conclusion    The RhoC-siRNA expression vector can effectively suppress RhoC expression in transfected hepatoma carcinoma cells. Although having no effect on the morphous and growth velocity of hepatoma carcinoma cells

it decreases the potentiality of cell invasion and cell metastasis

which may provide a novel applicable strategy for gene therapy on hepatocellular carcinoma.