Objective    To detect the expression changes and location of the mouse developmental regulation brain protein (Dbn1) in the developmental mouse brain.     Methods    Monoclonal antibodies against drebrin protein were used to assess Dbn1 by immunohistochemistry and Western blot. The paternal expression of Dbn1 in developmental mouse brain (E14

P1

P7 and adult) was initially investigated by Western blot. Dbn1 was shown at various developmental stages (E14

P1 and P7) as well as in adult in different brain area of developmental mouse brain by immunohistochemical.     Results    Dbn1 protein was detected in developmental brain but a little in adult brain by Western blot

high at E14

decreased at P1

gradually increased at P7 and lowest in adult. Immunohistochemistry confirmed as follows: Dbn1 expressed mostly in cortex

hippocampus and ependyma areas at E14

and the positive signal was distributed at cells border; The expression of Dbn1 was decreased at P1

mainly distributed at the verge of cells or dendrites; The peak expression of Dbn1 appeared at P7

Dbn1 located at nucleus of hippocampus and cortex

and the positive signal located within cytoplasm and dendrites; Only a little positive Dbn1 cells were found in adult mouse brain.     Conclusion    Dnb1 may be involved in regulating the differentiation and migration of neurons during the development of mouse brain.