GUO Yuan,LIU Yong-kang,LI Hong-tao,DU Ying,CHEN Guo-min.Condensation mechanism of aminoglucomannan with plasmid DNA[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(14):1378-1380.
GUO Yuan,LIU Yong-kang,LI Hong-tao,DU Ying,CHEN Guo-min.Condensation mechanism of aminoglucomannan with plasmid DNA[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(14):1378-1380.DOI：
Objective To study the condensation mechanism of aminoglucomannan (AGM) with plasmid DNA and some influencing factors in this process. Methods The polysaccharide-DNA complex was analyzed with agarose gel electrophoresis by observing the changes of DNA bands under various influencing conditions including different pH
ionic strength (NaCl concentration)
temperature and plasmid DNA sorts. Results The new bands appeared in the slow migration positions after reaction between AGM and plasmid DNA
and the changes of DNA bands content were dependent on AGM concentrations. The condensation degree could be effected by pH
temperature
and ionic strength. Conclusion Our study suggests that the AGM can be condensed with plasmid DNA in proper conditions.
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