Objective    To acquire specific polyclonal antibody of mouse IFIT1 (Interferon-induced protein with tetratricopetide repeats 1).     Methods    The single clone of pMAL-C2X-IFIT1

confirmed previously to highly express MBP-IFIT1 fusion protein

was cultivated and amplified. After ultrasonic fragmentation and centrifugation

the MBP-IFIT1 fusion protein was purified from the supernatant by using the Amylose Pre-packed column. Then

the MBP-IFIT1 fusion protein

mixed with Freund’s adjuvant

was injected into rabbits twice with two-week interval. The rabbit serum was collected

then its anti-IFIT1 titer was detected by ELISA

and its anti-IFIT1 specific reaction was detected by Western blot.     Results    The rabbit serum immunized with purified MBP-IFIT1 protein showed specific anti-IFIT1 ability

and its antibody titer was about 1∶12 800.      Conclusion    The purified MBP-IFIT1 fusion protein possesses favorable antigenicity

and highly specific polyclonal antibody of mouse IFIT1 can be obtained by using purified MBP-IFIT1 as antigen to immunize rabbit.