CHANG Qing,ZHAO Ze-wen,LI Ze-gui,LIANG Zhi-qing.Effect of PCB153 induced-apoptosis on mouse embryonic development[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(19):1838-1840.
CHANG Qing,ZHAO Ze-wen,LI Ze-gui,LIANG Zhi-qing.Effect of PCB153 induced-apoptosis on mouse embryonic development[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(19):1838-1840.DOI:
Objective To explore the effects of polychlorinated biphenyl congener (PCB
153
) an environmental estrogen
on embryonic development and its mechanism. Methods Mouse embryos of gestational day 8.5 were exposed to different doses of PCB
153
ex vitro. Post-implantation whole embryo culture (WEC) technique was used to investigate the effects of PCB
153
on growth and development. The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labelling (TUNEL) technique was used to detect apoptosis in embryonic tissue sections. Results PCB
153
had obvious effects on the development and growth of embryos in a dose-dependent manner; The ratio of apoptotic cells was increased with increased doses of PCB
153
. Conc
lusion PCB
153
-induced apoptosis is involved in mouse embryo anomalies.