Objective    To investigate the pharmacokinetics of a 12-amino-acid residue peptide of vascular endothelial growth factor (VEGF

125-136) in rabbits.     Methods    VEGF125-136 was indirectly labeled with

99

Tc

m

. After a single dose of 37 MBq of

99

Tc

m

-VEGF125-136 was injected intravenously in 6 rabbits

the radioactive concentration

s at different sampling times were determined. Then the plasma radioactive concentration-time curve was drawn and pharmacokinetics parameters were calculated with DAS computer program.      Results    The radiochemistry purity of

99

Tc

m

-VEGF125-136 was more than 97%

the radioactive concentration-time curve of

99

Tc

m

-VEGF125-136 was fitted to a three-compartment model with the main pharmacokinetic parameters as follows: t

1/2α

t

1/2β

and t

1/2γ

were (5.327±2.351)

(26.446±6.502) and (304.41±216.811) min respectively. CL

K

10

K

12

K

21

K

31

and K

13

were (4.00±1.00) ml/min

(0.047±0.034) min

-1

(40.65±68.54) min

-1

(0.111±0.058) min

-1

(0.111±0.058) min

-1

and (0.002±0.004) min

-1

respectively.     Conclusion    The pharmacokinetics is fitted to three compartment model in rabbits. VEGF125-136 can be cleared quickly from blood and its detention time in target tissue is long.