Objective     To establish an animal model of hepatic inflow occlusion with meso-caval shunt in Bama swines so as to evaluate the tolerance limit of normothermic hepatic inflow occlusion.      Methods     Eighteen Bama swines were divided into group A in which the hepatic blood inflow was occluded for 90 min and group B in which the hepatic blood inflow was blocked for 100 min. The animal survival rate

the changes of liver function and histopathology were observed.      Results     The animal survival

rate was significantly higher in group A than group B  (100% vs 44.4%

P0.05). Compared with the pre-ischemia value

ALT levels in the two groups on the 2

nd

and 3

rd

day postoperatively were significantly increased. On the 4

th

day postoperatively ALT recovered to normal level. ALT level was significantly higher in group B than group A on the 2

nd

and 3

rd

days postoperatively. AST in the two groups peaked at 1 h after reperfusion

and recovered to normal on the 3

rd

day postoperatively. AST was significantly higher in group B than group A at 1 h after reperfusion and on the 1

st

and 2

nd

days postoperatively. T-BIL and D-BIL levels in group A at ischemia 90 min were significantly higher than the pre-ischemia levels

and then decreased to the normal level. T-BIL

D-BIL levels in group B peaked after 100 min of ischemia

and returned to normal on the 5

th

day postoperatively. T-BIL and D-BIL levels in group B were significantly higher than group A at the end of ischemia

1 h after reperfusion and postoperative days 1-4. Hepatic injury was severer at 1 h after reperfusion in both groups. The hepatic tissue recovered to normal in group A on the 4

th

day postoperatively; however

hepatic necrosis was found in group B.      Conclusion     Ninety minutes is the tolerance limit for normothermic hepatic inflow occlusion in swine under conditions of meso-caval shunt.