Objective     To explore the relationship between the activation of glial cells and the change of excitatory amino acids (EAA) in the cornu dorsal medullae spinalis induced by pain due to chronic prostatitis.      Methods     The pain models of chronic prostatitis were induced by injecting complete Freund’s adjuvant and 3% carrageenan into the prostate of 40 rats. Another 20 rats served as normal control. Propentofylline was given with PE-10 in spinal cord of 20 rat models. The activation of astrocyte and microglia and Glu in the spinal cord was detected with immunohistochemistry and the change of excitatory amino acids was observed by amino acids analyzer.      Results     Activation of astrocyte and microglia was significantly increased in the models and significantly reduced in interfered groups. Glu was significantly expressed in cornu dorsal medullae spinalis

and Glu and aspartic acid (Asp) was significantly increased in the models and significantly reduced in interfered groups.      Conclusion     The activation of glial cells is an important reason for the change of excitatory amino acids in cornu dorsal medullae spinalis induced by pain due to chronic prostatitis of rats. Inhibitor of gliocyte activation will be a new way to treat the pain of chronic prostatitis.