Objective     To prepare and identify the mimovirus tumor vaccine based on survivin.      Methods     Under specific condition

peptide and DNA were condensed into like-virus particle

and the preparing parameters were optimized by assays of DNA gel retardation

DNase Ⅰ protection and identified by Western blotting. Antigen presentation was also studied by RMA-S/Kd cells.      Results     The vaccine was condensed into homogeneous particles at charge ratio of 2 in 87.5 mmol/L NaCl concentration

which could effectively mediate expression of the genes carried by vaccine particles.      Conclusion     The mimovirus tumor vaccine based on survivin was prepared successfully

providing a good foundation for the design of new tumor vaccine.