Objective     To investigate the effect of gadolinium chloride (GdCl

3

) on the H

22

experimental hepatocarcinogenesis in mice.      Methods     Totally 80 mice were inflicted to experimental hepatoma by implanting H

22

cells to their liver lobes

and then equally and randomly divided into experimental hepatoma group (B) and GdCl

3

pretreatment group (10 mg/kg

C). Another 40 mice served as normal control group (A). Ten mice from every group were killed respectively 7

14

and 28 d after implantation. The left 10 mice were used for recording survival time and measuring the mass weight. Hepatic patholog

ical histology was observed

and the expression of TNF-α was detected by ELISA and RT-PCR.       Results     ①Survival time was obviously higher in group B than in group C (P0.01)

and the tumor weight percentage was obviously lower in group B than in group C (P0.05). ②Normal control group had normal histological structure around central vein in the liver and hepatic cells. Group B and C showed disordered cell array

atypia of organization

enlarged and darkened nuclei

unobvious apoptosis

and a few inflammatory cells infiltrating around the liver cancer tissue. ③Compared with group C

the serum TNF-α was increased at each time period in group B (P0.05). ④RT-PCR semiquantitative analysis showed TNF-α mRNA was higher at each time period in group B than in group C.      Conclusion     GdCl

3

can inhibit effectively the immunity activity of Kupffer cells

thereby

reduce the H

22

experimental hepatocarcinogenesis in mice.