GAN Xiao-qin,YAO Zhen-wei.Effect of imitated CO2 pneumoperitoneum on expressions of Survivin and Bcl-2 in human cervical carcinoma cell line[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(13):1323-1325.
GAN Xiao-qin,YAO Zhen-wei.Effect of imitated CO2 pneumoperitoneum on expressions of Survivin and Bcl-2 in human cervical carcinoma cell line[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(13):1323-1325.DOI:
pneumoperitoneum on the anti-apoptotic capability of human cervical carcinoma cell line (CaSki). Methods The imitated CO
2
pneumoperitoneum was established in vitro. After CaSki cells were exposed to CO
2
pneumoperitoneum at 0
7 or 14 mmHg for 1
2
3
4 h
the expressions of Survivin mRNA and Bcl-2 protein were examined by RT-PCR and Western blot respectively. The untreated CaSki cells served as control. Results Under 7 or 14 mmHg
the expressions of Survivn and Bcl-2 had an increasing tendency w
ith the exposure time prolongation. Within the same exposure time
the expressions of Survivin and Bcl-2 under 7 mmHg were significantly higher than those under 0 or 14 mmHg or in control group. Conclusion Under certain pressure and within certain exposure time
CO
2
pneumoperitoneum could improve anti-apoptotic capability of human cervical carcinoma cells
which may be one reason for the promoting effect of carbon dioxide pneumoperitoneum on malignant cells growth.