Objective    To study the therapy for xenograft human bile duct cancer in mice mediated by adenovirus containing Apoptin gene.     Methods    Subcutaneous human bile duct cancer was established in nude mice. Variations of tumor volume and histomorphology

side effects were observed after intratumoral injection of adenovirus containing Apoptin gene. Finally the mice were sacrificed for calculating the ratio of antitumor.     Results    Twelve days after treatment

the mean volume of the xenograft human bile duct cancer in the group of intratumoral injection of adenovirus containing Apoptin gene was (92.31±28.31) mm

which was reduced significantly compared with that of adenovirus infection without apoptin gene(288.86±113.13)mm and control group(344.86±113.87)mm. The ratio of antitumor was 72.10%

which was significantly higher than that in control group（11.9％）. During the whole experimental course

no side effect was observed. The histological results demonstrated that the reduction of tumor growth was the result of apoptosis in bile duct cells

which was reduced by transfection of Apoptin gene.     Conclusion    The adenovirus vectors containing Apoptin gene may constitute a safe tool for the treatment of cholangiocarcinoma.