不同来源茶树油抑制LPS诱导TNF-α释放的体外实验

王懿; 王振维

您当前的位置：

首页 >

文章列表页 >

不同来源茶树油抑制LPS诱导TNF-α释放的体外实验

论著 | 更新时间：2026-01-09

- 不同来源茶树油抑制LPS诱导TNF-α释放的体外实验
- Inhibition effect on LPS-induced TNF-α release by homegrown and Australian Melaleuca alternifolia (Tea Tree) oil
- 陆军军医大学学报 2007年29卷第4期页码：324-327
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R282.71;R364.5;R392.5
- 纸质出版：2007
- 稿件说明：
移动端阅览
王懿,王振维.不同来源茶树油抑制LPS诱导TNF-α释放的体外实验[J].陆军军医大学学报(原第三军医大学学报),2007,29(04):324-327.

　WANG Yi,WANG Zhen-wei.Inhibition effect on LPS-induced TNF-α release by homegrown and Australian Melaleuca alternifolia (Tea Tree) oil[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(04):324-327.
王懿,王振维.不同来源茶树油抑制LPS诱导TNF-α释放的体外实验[J].陆军军医大学学报(原第三军医大学学报),2007,29(04):324-327. DOI：

　WANG Yi,WANG Zhen-wei.Inhibition effect on LPS-induced TNF-α release by homegrown and Australian Melaleuca alternifolia (Tea Tree) oil[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(04):324-327. DOI：

摘要

目的比较国产和进口茶树油（tea tree oil，TTO）对脂多糖(lipopolysaccharide， LPS)诱导炎症的抑制作用。方法四唑盐（MTT）比色实验筛选无细胞毒性作用的TTO溶液浓度；各浓度TTO水溶液按量效和时效关系预处理细胞后，测定LPS激活RAW264.7细胞分泌肿瘤坏死因子-α(tumor necrotic factor-alpha， TNF-α)的浓度，以观察国产和进口TTO水溶液对LPS诱导炎症的抑制效果。结果浓度低于0.001%的国产和进口TTO溶液对细胞无毒性作用；0.000 125%～0.001%进口TTO和0.000 25%～0.001%国产TTO各浓度组均能非常显著地抑制LPS诱导细胞释放TNF-α；0.000 25%进口和国产TTO分别在3 h和6 h内对细胞有显著的保护作用。结论国产和进口TTO对LPS诱导的炎症均有显著抑制效果。

Abstract

Objective To compare the inhibition of the homegrown and Australian tea tree oil (TTO) solution on TNF-α release from RAW264.7 cells induced by LPS. Methods MTT assay was used to measure the effects of TTO on viability of RAW264.7 in vitro. RAW264.7 cells were pretreated with two kinds of TTO solution in the dose- and time-dependent manners

then stimulated by LPS. TNF-α in the supernate was tested by ELISA. Results In vitro

two kinds of TTO (＜0.001%) showed no cytotoxicity to RAW264.7 cells. Australian TTO (0.000 125%-0.001%) and homegrown TTO (0.000 25%-0.001%) significantly suppressed LPS-induced TNF-α release

respectively. 0.000 25% Australian TTO pretreating for 3 h and homegrown TTO pretreating for 6 h showed significant protections against the stimulations of LPS

respectively. Conclusion In vitro

two kinds of TTO have the obvious inhibitory action

and there is no significant difference between Australian and homegrown TTO.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

柚皮苷改善脂多糖诱导的小鼠抑郁样行为的作用研究

非侵入式静电场刺激对脂多糖诱导神经认知障碍小鼠的改善作用

冠蛋白-1通过调控CD14的表达影响巨噬细胞极化

GSDMD介导的细胞焦亡对小鼠骨骼肌肌肉萎缩的影响及机制研究

lncRNA NKILA靶向miR-1236-3p对脂多糖诱导的支气管上皮细胞损伤的影响