Objective     To explore the ability of revascularization of big section deproteinized bone (DPB) allograft combining calcium phosphate cement (CPC) with recombinant human vascular endothelial growth factor (rhVEGF) and bone morphogenetic protein-2 (rhBMP-2).      Methods     Totally 36 adult hybrid dogs were inflicted to demi-articular defect models by exsecting 30 mm right femur inferior extremity near articular surface，and then randomly divided into 3 groups

group A

receiving material CPC/rhVEGF/rhBMP-2/DPB transplantation into the demi-articular defect

group B

material rhVEGF/rhBMP-2/DPB transplantation

and  group C with simple material DPB transplantation. At 4

8

12 and 16 weeks after operation

vasculogenesis and new bone formation were assessed by X-ray examination

histological examination

transmission electron microscopy

vas capillare analysis by vaso-filling with ink and radionuclide bone imaging examination (ECT).      Results     The bone formation and vascularization was significantly superior in group A to that in group B and C. The results of vaso-filling examination with ink and arterial perfusion of ECT examination were both significantly superior to that in group B and C (P0.01). But there was no significant difference between group B and C.      Conclusion     The materials CPC/rhVEGF/rhBMP-2/DPB can promote the bone formation and early formation of local blood vessels

even achieve bone healing and become autologous tissue.