Objective    To clarify the effect of NO on hypoxic myocardium in rats.     Methods    Myocardial cells from neonatal Wistar rats aged 1-2 days cultured for 4-6 days

then pretreated with GSNO (30

200

4 000 μmol/L) were exposed to normoxia (N) or hypoxia (H)(1%O

2

5%CO

2

and 94%N

2

) for 4 h. The NO content and LDH in the supernatant were detected.     Results    NO of 1.43

7.2 and 70 μmol/L were detected respectively in the supernatant of hypoxic myocardial cells treated by GSNO (30

200 and 4 000 μmol/L). Compared with normoxic group

LDH activity increased and myocardial cell activity decreased markedly in H+G30 and H+G4000 groups but les

s injury was observed in H+G200 group.     Conclusion    NO of certain concentration can markedly abate myocardial cell injury induced by hypoxia.