n-6/n-3多不饱和脂肪酸对乳腺癌大鼠乳腺癌组织CYP17和CYP19表达的影响

王静; 糜漫天; 韦娜; 王斌

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n-6/n-3多不饱和脂肪酸对乳腺癌大鼠乳腺癌组织CYP17和CYP19表达的影响

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- n-6/n-3多不饱和脂肪酸对乳腺癌大鼠乳腺癌组织CYP17和CYP19表达的影响
- Effect of n-6/n-3 PUFA dietary of different constituent ratio on expressions of CYP17, CYP19 mRNA in rat breast tissues of MNU-induced breast cancer
- 陆军军医大学学报 2007年29卷第8期页码：674-677
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  中图分类号： R151.2;R394.3;R737.9
- 纸质出版：2007
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王静,糜漫天,韦娜,王斌.n-6/n-3多不饱和脂肪酸对乳腺癌大鼠乳腺癌组织CYP17和CYP19表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):674-677.

　WANG Jing,MI Man-tian,WEI Na,WANG Bin.Effect of n-6/n-3 PUFA dietary of different constituent ratio on expressions of CYP17, CYP19 mRNA in rat breast tissues of MNU-induced breast cancer[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):674-677.
王静,糜漫天,韦娜,王斌.n-6/n-3多不饱和脂肪酸对乳腺癌大鼠乳腺癌组织CYP17和CYP19表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(08):674-677. DOI：

　WANG Jing,MI Man-tian,WEI Na,WANG Bin.Effect of n-6/n-3 PUFA dietary of different constituent ratio on expressions of CYP17, CYP19 mRNA in rat breast tissues of MNU-induced breast cancer[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(08):674-677. DOI：

摘要

目的探讨不同n-6/n-3多不饱和脂肪酸(PUFA)膳食构成比对MNU诱导的乳腺癌大鼠血清雌二醇水平及乳腺组织CYP17、CYP19 mRNA表达的影响。方法雌性SD大鼠65只随机分为5组，即n-6 PUFA组、10∶1 n-6/n-3组、5∶1 n-6/n-3组、1∶1 n-6/n-3组和正常对照组，前4组以50 mg/kg MNU单次腹腔注射诱导大鼠乳腺肿瘤发生，正常对照组注射等体积无菌生理盐水。给药后立即分组喂养不同饲料，并在8周和18周时经阴道涂片证实间情期，股动脉取血液并剖取乳腺组织，采用放射免疫法检测血清雌二醇水平，利用RT-PCR技术检测乳腺组织中CYP17、CYP19 mRNA表达状况。结果 4组乳腺癌大鼠血清雌二醇水平及乳腺组织中CYP17、CYP19的表达较正常对照组均有所升高，其中 n-6组最高，其余各组随n-6/n-3值减小而下降，1∶1 n-6/n-3组显著低于n-6组。结论不同n-6/n-3 PUFA膳食构成比对MNU诱导的乳腺癌大鼠血清雌二醇水平及乳腺组织CYP17、CYP19 mRNA的表达具有不同影响，1∶1 n-6/n-3 PUFA膳食构成比能有效抑制乳腺癌大鼠血清雌二醇水平及乳腺组织CYP17、CYP19 mRNA表达的升高。

Abstract

Objective To observe the effect of n-6/n-3 polyunsaturated fatty acid dietary of different constituent ratio on the serum level of estradiol and expressions of CYP17

CYP19 in MNU-induced rat breast cancer tissues. Methods Totally 65 female SD rats were randomly divided into 5 groups: n-6 PUFA

10∶1 n-6/n-3 PUFA

5∶1 n-6/n-3 PUFA

1∶1 n-6/n-3 PUFA and normal control. Except control group receiving physiological saline

all rats were given a single intraperitoneal injection of MNU (50 mg/kg) and switched to different diets containing fatty acids mentioned above. After 8 and 18 weeks

the serum level of estradiol and expressions of CYP17

CYP19 in the breast tissues of different groups were determined by RIA and RT-PCR. Results The serum level of estradiol and expressions of CYP17

CYP19 in rat breast cancer tissues were higher than normal control

highest in n-6 group and gradually decreased with the ratio of n-6/n-3 decreasing. Conclusion n-6/n-3 PUFA dietary of different constituent ratio can diversely influence the serum level of estradiol and expressions of CYP17

CYP19 in MNU-induced rat breast cancer tissues. n-6/n-3 PUFA dietary with constituent ratio of 1∶1 can significantly inhibit the increased serum level of estradiol and increased expressions of CYP17

CYP19 in breast cancer tissues.

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