Objective      To construct recombinant adenovirus carrying human DeltaNp73α cDNA.       Methods      Human DeltaNp73α cDNA was cloned into adenovirus transfer vector pAdTrack-CMV. The obtained plasmid

pAdTrack-CMV-DeltaNp73α

was then linearized with PmeⅠ and transformed into E. coli. strain BJ5183 containing pAdeasy-1

the viral DNA plasmid

for homologous recombination. The adenoviral recombinant was then cleaved with PacⅠ and transfected into 293T cells to produce viral particles. The virus was then transfected into dentritic cells

and the expression of DeltaNp73α was proved by GFP expression and Western bl

otting.       Results      Recombinants were confirmed right by restriction enzyme analysis

PCR on viral lysate and expression of green fluorscence protein. A band of 67×10

3

was observed by Western blotting as expected.       Conclusion      The recombinant adenovirus carrying human DeltaNp73α cDNA is successfully constructed

paving the way for further research about its high expression in interested cells.