Objective     To explore the promoting effects of IL-2 and IL-10 on Treg proliferation in vitro

and to compare the inhibiting effects of the proliferated Treg on CTL with those of the enriched Treg.      Methods     After IL-2 or IL-10 was added into the culture system to stimulate Treg proliferation

the Treg cells were counted at 48

96 and 144 h to determine the cytokines’ efficacious doses in vitro. After collecting the proliferated Treg

we compared the inhibiting effects of the proliferated Treg on CTL with those of the enriched Treg.      Results     IL-2 and IL-10 induced Treg proliferation in vitro significantly. IL-2(100 ng/ml) increased the number of Treg cells by 10%

18% and 20% in vitro at 48

96 and 144 h after stimulation. IL-10 (150 ng/ml) provided the same inducing effects as IL-2

proving less capable. The proliferated Treg had the same efficacy in inhibiting CTL functions as the enriched Treg (P0.05).      Conclusion     IL-2 is superior to IL-10 for Treg proliferation in vitro. The proliferated Treg is as efficacious as MACS enriched Treg in inhibiting CTL function.