Objective    To investigate the protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis (Em) and against challenge of Em protoscolexes.      Methods    BALB/C mice were subcutaneously or intranasally vaccinated with mixed recombinant BCG vaccines respectively. Eight weeks later

the immunized mice were challenged with Em protoscolexes. On the 18

th

week after vaccination

the mice were killed to count the rate of reduced

alveolar echinococcus weight and to examine the levels of IgG and its subclasses

IgE; the spleen cells were separated to measure subsets of CD4

+

and CD8

+

T cells by FACsort. Blank vector

BCG and PBS served as control.     Results    In the immunized groups

the rate of reduced alveolar echinococcus weight was 45.29%-76.47%; the levels of IgG

IgG2a

IgG2b and IgE increased apparently while IgG1 and IgG3 decreased remarkably; CD4

+

subset and the ratio of CD4

+

/CD8

+

increased obviously

but CD8

+

subset had no change.     Conclusion    Intranasal vaccination with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine is a good way for immunization. IgG

IgG2a

IgG2b

IgE and CD4

+

T cell subset may play an important role in protecting against challenge of Em protoscolexes.