Objective    To further determine their possible synergistic effect on accelerating wound healing

adenovirus vector containing recombinant human hPDGF-A and hBD

2

genes was constructed and the expression of exogenous genes in transformed mesenchymal stem cells derived from rat bone marrow was observed.     Methods    By putting IRES in the middle of hPDGF-A and hBD

2

these two genes were expected to be expressed individually. The

shuttle vector was named as pAdTrack-hPDGF-A-IRES

2

-hBD

2

which homologously recombinated with Adeasy-1 in BJ5183 cells and formed the mammalian expression vector pAdeasy-hPDGF-A-IRES

2

-hBD

2

. Furthermore

the recombinant vector was packaged in 293 cells into infectious recombinant adenovirus

which were used to infect BMSCs. The expression of hPDGF-A and hBD

2

in BMSCs was detected by RT-PCR.     Results    We successfully constructed recombinant adenovirus vector that simultaneously expressed hPDGF-A and hBD

2

. The expressions of hPDGF-A and hBD

2

were confirmed by RT-PCR on transformed BMSCs.     Conclusion    The established BMSCs that overexpressed hPDGF-A and hBD

2

provide a new strategy of combining cell therapy and gene therapy to promote wound healing

especially the chronic one.