Objective    To detect whether mice bone marrow mesenchymal stem cells (BMSCs) can contribute to the regeneration of hepatocytes in bone marrow chimeric mice.     Methods    Female recipient mice (C57BL/6J) underwent whole body gamma-ray irradiation with a dose of 10 Gy to ablate their bone marrow

followed by immediate tail vein injection of BMSCs isolated from male GFP transgenic mice. Animals were killed at different phase points: 1 week

1 month

and 3 months. Using fluorescence microscope we directly observed GFP-positive cells in the liver frozen sections

and we also prepared the parafilm sections to detect the GFP-positive cells and the coexpression of GFP and Alb

CK18 by immunohistochemistry and immunofluorescence respectively.     Results    We found numerous GFP-positive cells in recipient mice liver at 1 week after BMSCs transplantation

some at 1 month and seldom at 3 months. There were some cells coexpressing GFP and Alb

CK18 at all the phase points.     Conclusion    Allotype BMSCs can differentiate into Alb and CK18 positive hepatocytes in bone marrow chimeric mice

which will become an ideal cell resource for liver tissue project.