LU Wei-hong,TIAN Huai-jun.Effects of hypoxia on morphology and survival of rat Sertoli cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(01):65-67.
LU Wei-hong,TIAN Huai-jun.Effects of hypoxia on morphology and survival of rat Sertoli cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(01):65-67.DOI:
Objective To explore the effect of hypoxia on cultured rat Sertoli cells in vitro. Methods In a Sertoli-germ cells co-culture system
the effect of hypoxia on the morphological characteristics of Sertoli cells were observed
including the number of Sertoli cells
the cytoskeleton changes after Coomassie brilliant blue staining and the detachment of germ cells. Results The loosing and retracting of cytoskeleton in Sertoli cells was found. Both survival rate of Sertoli cells and detachment of germ cells showed time-response relation. Conclusion Hypoxia damages Sertoli cells.