Objective     To clone mouse FasL (mFasL) full length cDNA and construct eukaryotic expression vector

and to detect its expression in dendritic cells.      Methods     RT-PCR and TA cloning technique were used to clone the mFasL full length cDNA from mouse activated T lymphocytes

and then eukaryotic expression vector pIRES2EGFP containing mFasL cDNA was constructed. The positive  recombinants pIRES2EGFP-mFasL were transfected into dendritic cells (DC)

the expression of mFasL mRNA was detected by RT-PCR and the expression of mFasL protein was assayed by immunofluorescence and Western blotting.      Results     The cloned full reading frame of mFasL cDNA was in coincidence with the sequence registered in GenBank. In the DC after transfection of pIRES2EGFP-mFasL

the expressions of mFasL mRNA and mFasL protein were detected.      Conclusion     The mFasL cDNA was cloned and constructed into the eukaryotic expression vector successfully

and mFasL gene could be expressed efficiently in DC transfected by pIRES2EGFP-mFasL.