Objective     To acquire NF-κB p65 TAD (transcription activation domain) and construct its eukaryotic expression vector and express it in endothelial cells.      Methods     Human umbilical vein endothelial cells (HUVECs) were cultured and total RNA was extracted. The p65 TAD gene was amplified by RT-PCR. After sequencing

the p65 TAD gene was inserted into the eukaryotic expression vector pEGFP-N1 with the green fluorescence protein

named pEGFP-N1-p65 TAD. pEGFP-N1-p65 TAD was transfected into HUVECs and its expression was observed under fluorescence microscope and analyzed by Western blotting.      Results     p65 TAD (288-548) was cloned successfully. The constructed plasmid including p65 TAD gene was identical to the designed. p65 TAD gene was expressed successfully in HUVECs.      Conclusion     The construction of eukaryotic expression vector including p65 TAD gene and its expression in HUVECs are very successful.