Objective     To establish scaffold materials of deproteinized heterogeneous tissue engineering bone.      Methods     Deproteinized bones were made through a series of physicochemical treatment from pig ribs and were analyzed with histology

scanning electron microscopy

infrared spectrum

X-ray diffraction analysis

X-ray energy scattering analysis

microdose Kjeldahl determination and mechanical analysis.      Results     Stain of interstitial collagen fibers was positive and mucin stain was negative in deproteinized bone

but stain of interstitial collagen fibers and mucin were all positive in fresh bone. Deproteinized bone maintained natural network pore system

its pore size was (472.51±7.02) μm

its porosity was (78.15±6.45)%. Infrared spectrum showed collogen in deproteinized bone. Fresh and deproteinized bone were all of curve style atlas of hydroxyapatite

their ratio of calcium to phosphors was (1.71±0.95) and (1.68±0.76) (P0.05)

and their protein content was (26.6±2.23)% and (19.1±2.14)% (P0.05). The compression destruction load and destruction maximum load of deproteinized bone were of no significant difference with those of fresh bone (P0.05)

except for high elastic modulus.      Conclusion     Physicochemical property and mechanical strength of scaffold materials of established deproteinized tissue engineering bone meet request of ideal scaffold materials. Its immunogenicity should be further observed.