Objective     To investigate the effect of bFGF and MSCs autografting on expression of PCNA and Ⅷ-R Ag of porcine skin defect wound.      Methods     Six male minipigs were used to produce 48 full-thickness skin wounds

each wound area about 2.54 cm

2

on both sides of the back (8 wounds per pig). These 48 wounds were randomly treated with bFGF

MSCs

bFGF+MSCs

and normal saline (12 wounds for each treatment). The tissue from the wounds was harvested o

n day 3

7

14 after surgery

then used for HE staining and immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and factor Ⅷ related antigen (Ⅷ-R Ag).      Results     Granulation tissue was presented in all groups on day 3 and 7 after wound

most abundant in the wounds treated with bFGF+MSCs

as well as blood capillary formation. PCNA expression increased after skin injury

reached the peak on day 7

most in the granulation tissue

and lasted to day 14

then began to decrease. On day 3

7

14 after skin injury

PCNA expression was the most highest in the wounds treated with bFGF+MSCs. Ⅷ-R Ag-positive cells in the wounds treated with bFGF+MSCs were significantly more than that of the other three treatments (P0.05).       Conclusion     bFGF in coordination with MSCs can promote the expression of PCNA and Ⅷ-R Ag of wounds and accelerate granulation tissue growth.