Effect of morin on alveolar bone resorption in periodontitis mice by regulating the SIRT1/PGC-1α/Nrf2 pathway

DING Chunyan; WANG Ruijuan; WANG Yijun; MENG Liying; FANG Guanglin

doi:10.6039/j.issn.1001-0408.2026.07.13

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Effect of morin on alveolar bone resorption in periodontitis mice by regulating the SIRT1/PGC-1α/Nrf2 pathway

更新时间：2026-07-16

- Effect of morin on alveolar bone resorption in periodontitis mice by regulating the SIRT1/PGC-1α/Nrf2 pathway
- ZHONGGUO YAOFANG Vol. 37, Issue 7, Pages: 902-907(2026)
- 作者机构：
  
  1.天津医科大学附属宝坻医院口腔科，天津　301800
  2.天津市宝坻区海滨医院口腔科，天津　301800
  3.天津市宝坻区周良卫生院口腔科，天津　301800
  4.天津市宝坻区郝各庄医院口腔科，天津　301800
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2026.07.13
  CLC： R965;R285
- Received：16 December 2025，
  
  Revised：2026-03-06，
  
  Accepted：09 March 2026，
  
  Published：15 April 2026
- 稿件说明：
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丁春燕,汪瑞娟,王毅军,等.桑黄素调控SIRT1/PGC-1α/Nrf2通路对牙周炎小鼠牙槽骨吸收的影响 [J].中国药房,2026,37(07):902-907.

DING Chunyan,WANG Ruijuan,WANG Yijun,et al.Effect of morin on alveolar bone resorption in periodontitis mice by regulating the SIRT1/PGC-1α/Nrf2 pathway[J].ZHONGGUO YAOFANG,2026,37(07):902-907.
丁春燕,汪瑞娟,王毅军,等.桑黄素调控SIRT1/PGC-1α/Nrf2通路对牙周炎小鼠牙槽骨吸收的影响 [J].中国药房,2026,37(07):902-907. DOI： 10.6039/j.issn.1001-0408.2026.07.13.

DING Chunyan,WANG Ruijuan,WANG Yijun,et al.Effect of morin on alveolar bone resorption in periodontitis mice by regulating the SIRT1/PGC-1α/Nrf2 pathway[J].ZHONGGUO YAOFANG,2026,37(07):902-907. DOI： 10.6039/j.issn.1001-0408.2026.07.13.

摘要

目的

基于沉默信息调节因子1/过氧化物酶体增殖物激活受体γ共激活因子1α/核转录因子红系2相关因子2（SIRT1/PGC-1α/Nrf2）通路，研究桑黄素对牙周炎小鼠牙槽骨吸收的影响及机制。

方法

将小鼠随机分为对照组、模型组、桑黄素组（40 mg/kg）、SRT1720（SIRT1激活剂）组（5 mg/kg）、桑黄素+EX527（SIRT1抑制剂）组（40 mg/kg桑黄素+7.5 mg/kg EX527），每组18只。除对照组外，其余各组小鼠均通过丝线结扎法建立牙周炎模型。建模成功后，各组小鼠灌胃或腹腔注射相应药液/生理盐水，每天给药1次，连续2周。末次给药后，检测小鼠血清中肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-1β、IL-6、IL-10水平；测量小鼠釉牙骨质界与牙槽骨嵴顶的距离，并计算骨体积分数和骨矿物质密度；观察小鼠牙周组织病理学形态；检测小鼠牙周组织中破骨细胞数；检测小鼠牙周组织中核因子κB受体激活蛋白配体（RANKL）、骨保护素（OPG）mRNA表达水平和丙二醛（MDA）、超氧化物歧化酶（SOD）水平以及SIRT1、PGC-1α、Nrf2蛋白表达水平。

结果

与模型组比较，桑黄素组、SRT1720组小鼠牙槽骨吸收和牙周组织炎症细胞浸润现象有所改善；血清中TNF-α、IL-1β、IL-6水平，釉牙骨质界与牙槽骨嵴顶的距离，牙周组织中破骨细胞数、RANKL mRNA表达水平、MDA水平均显著降低/缩短/减少（

＜0.05）；血清中IL-10水平，骨体积分数和骨矿物质密度，牙周组织中OPG mRNA表达水平、SOD水平和SIRT1、PGC-1α、Nrf2蛋白表达水平均显著升高（

＜0.05）。与桑黄素组比较，桑黄素+ EX527组小鼠上述病理改变明显加重，定量指标水平均显著逆转（

＜0.05）。

结论

桑黄素可能通过激活SIRT1/PGC-1α/Nrf2通路，减轻炎症反应和氧化应激反应，从而抑制牙周炎小鼠牙槽骨吸收。

Abstract

OBJECTIVE

To investigate the effect and mechanism of morin on alveolar bone resorption in periodontitis mice based on the silent information regulator 1 （SIRT1）/peroxisome proliferator-activated receptor γ coactivator-1α （PGC-1α）/nuclear factor-erythroid 2-related factor 2 （Nrf2） pathway.

METHODS

The mice were randomly divided into control group， model group， morin group （40 mg/kg）， SRT1720 （SIRT1 activator） group （5 mg/kg）， and morin+EX527 （SIRT1 inhibitor） group （40 mg/kg morin+7.5 mg/kg EX527）， with 18 mice in each group. Except for control group， mice in other groups were subjected to silk ligation to establish periodontitis model. After successful modeling， mice in each group were treated with corresponding medicinal solutions or normal saline intragastrically or intraperitoneally， once a day， for two consecutive weeks. After the last medication， serum levels of tumor necrosis factor-α （TNF-α）， interleukin （IL）-1β， IL-6 and IL-10 were measured. The distance between the cementoenamel junction and alveolar bone crest was determined， and bone volume fraction and bone mineral density were calculated. Pathological changes of periodontal tissue were observed， and the number of osteoclasts was measured. mRNA expressions of receptor activator of nuclear factor-κB ligand （RANKL） and osteoprotegerin （OPG） in periodontal tissue， the levels of malondialdehyde （MDA） and superoxide dismutase （SOD） as well as protein expressions of SIRT1， PGC-1α， and Nrf2 were determined.

RESULTS

Compared with model group， the alveolar bone resorption and inflammatory cell infiltration in the periodontal tissues of mice were improved in morin group and SRT1720 group. The serum levels of TNF-α， IL-1β and IL-6， the distance between cementoenamel junction and alveolar bone crest， the number of osteoclasts in periodontal tissue， RANKL mRNA expression and the MDA level were decreased， shortened and reduced significantly （

＜0.05）； however， serum level of IL-10， bone volume fraction and bone mineral density， OPG mRNA expression in periodontal tissue， SOD level and protein expressions of SIRT1， PGC-1α and Nrf2 were increased significantly （

＜0.05）. Compared with morin group， the above pathological changes were significantly aggravated in the morin+EX527 group； and the levels of quantitative indicators were markedly reversed （

＜0.05）.

CONCLUSIONS

Morin may inhibit alveolar bone resorption in periodontitis mice by activating the SIRT1/PGC-1α/Nrf2 pathway to reduce inflammatory reaction and oxidative stress.

关键词

Keywords

references

LI S H ， YANG W R ， LI A L ， et al . Protective effect of Nrf2 in periodontitis：a preclinical systematic review and meta-analysis [J ] . Arch Oral Biol ， 2023 ， 151 ： 105713 .

SADEK K M ， EL MOSHY S ， RADWAN I A ， et al . Molecular basis beyond interrelated bone resorption/regeneration in periodontal diseases：a concise review [J ] . Int J Mol Sci ， 2023 ， 24 （ 5 ）： 4599 .

张洋，宋磊，吴芷芸 . 复方黄芩含漱液对慢性牙周炎患者牙周临床指数的影响 [J ] . 锦州医科大学学报， 2025 ， 46 （ 4 ）： 64 - 67 .

HASHIM N T ， BABIKER R ， CHAITANYA N C S K ， et al . New insights in natural bioactive compounds for periodontal disease：advanced molecular mechanisms and therapeutic potential [J ] . Molecules ， 2025 ， 30 （ 4 ）： 807 .

SHAO Y ， CHEN Y F ， LAN X ， et al . Morin regulates M1/M2 microglial polarization via NF-κB p65 to alleviate vincristine-induced neuropathic pain [J ] . Drug Des Devel Ther ， 2024 ， 18 ： 3143 - 3156 .

PARK H J ， PARK J N ， YOON S Y ， et al . Morin disrupts cytoskeleton reorganization in osteoclasts through an ROS/SHP1/c-src axis and grants protection from LPS-induced bone loss [J ] . Antioxidants ， 2022 ， 11 （ 5 ）： 963 .

SHI Y F ， YE L ， SHEN S W ， et al . Morin attenuates osteoclast formation and function by suppressing the NF-κB，MAPK and calcium signaling pathways [J ] . Phytother Res ， 2021 ， 35 （ 10 ）： 5694 - 5707 .

WAN J ， MA T ， JIN Y ， et al . The effects of morin on bone regeneration to accelerate healing in bone defects in mice [J ] . Int J Immunopathol Pharmacol ， 2020 ， 34 ： 2058738420962909 .

伍栋，习梅 . 炎症微环境下SIRT1促进牙周膜干细胞成骨分化的作用及机制研究 [J ] . 实用口腔医学杂志， 2023 ， 39 （ 2 ）： 218 - 222 .

CHEN J S ， ZHANG Y R ， GAO J ， et al . Sirtuin 3 de- ficiency exacerbates age-related periodontal disease [J ] . J Periodontal Res ， 2021 ， 56 （ 6 ）： 1163 - 1173 .

TOSSETTA G ， FANTONE S ， TOGNI L ， et al . Modulation of NRF2/KEAP1 signaling by phytotherapeutics in periodontitis [J ] . Antioxidants ， 2024 ， 13 （ 10 ）： 1270 .

漆美瑶，祁星颖，周欣奕，等 . 大麻二酚联合米诺环素对牙周炎治疗作用的实验研究 [J ] . 国际口腔医学杂志， 2024 ， 51 （ 4 ）： 392 - 400 .

QIU L ， YAN C Q ， YANG Y ， et al . Morin alleviates DSS-induced ulcerative colitis in mice via inhibition of inflammation and modulation of intestinal microbiota [J ] . Int Immunopharmacol ， 2024 ， 140 ： 112846 .

ZHANG X S ， XIE H B ， LIU Z B ， et al . HMGB 1 acetylation mediates trichloroethylene-induced immune kidney injury by facilitating endothelial cell-podocyte communication [J ] . Ecotoxicol Environ Saf ， 2023 ， 259 ： 115042 .

魏雪乔，张建影，黄娟，等 . 基于SIRT1/PGC-1α通路探讨黄芪补肾活血汤对糖尿病肾病大鼠的保护作用及机制 [J ] . 陕西中医， 2025 ， 46 （ 8 ）： 1036 - 1041 .

YIN Y ， MA Z Y ， SHI P L . Nootkatone mitigates periodontal inflammation and reduces alveolar bone loss via Nrf2/HO-1 and NF-κB pathways in rat model of periodontitis [J ] . Folia Histochem Cytobiol ， 2024 ， 62 （ 3 ）： 145 - 153 .

刘昌玲，陈耀武，魏晓莹，等 . 慢性牙周炎患者血清CGRP、PGE2、CCL20与牙周临床指标和Th17/Treg失衡的相关性分析 [J ] . 现代生物医学进展， 2023 ， 23 （ 14 ）： 2745 - 2749 .

ZHANG Z J ， SONG J H ， KWON S H ， et al . Pirfenidone inhibits alveolar bone loss in ligature-induced periodontitis by suppressing the NF-κB signaling pathway in mice [J ] . Int J Mol Sci ， 2023 ， 24 （ 10 ）： 8682 .

WANG L T ， CHEN L R ， CHEN K H . Hormone-related and drug-induced osteoporosis：a cellular and molecular overview [J ] . Int J Mol Sci ， 2023 ， 24 （ 6 ）： 5814 .

SI Y H ， LI Y ， GU K ， et al . Icariin ameliorates osteoporosis in ovariectomized rats by targeting Cullin 3/Nrf2/OH pathway for osteoclast inhibition [J ] . Biomed Pharmacother ， 2024 ， 173 ： 116422 .

王朝晨，张浩，崔健，等 . 盐酸氟桂利嗪对脑出血大鼠氧化应激、炎症因子表达以及神经功能的影响及机制探索 [J ] . 现代生物医学进展， 2025 ， 25 （ 3 ）： 419 - 427，467 .

PAKSOY T ， USTAOĞLU G ， ŞEHIRLI A Ö ， et al . Effect of bromelain on periodontal destruction and alveolar bone in rats with experimental periodontitis [J ] . Int Immunopharmacol ， 2023 ， 121 ： 110446 .

XIE S C ， JIANG X L ， DOYCHEVA D M ， et al . Activation of GPR39 with TC-G 1008 attenuates neuroinflammation via SIRT1/PGC-1α/Nrf2 pathway post-neonatal hypoxic-ischemic injury in rats [J ] . J Neuroinflammation ， 2021 ， 18 （ 1 ）： 226 .

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