Study on the inhibitory effect and mechanism of Modified qifang weitong granules on gastric cancer

CHEN Xinyuan; WU Chengting; XIONG Changzhou; WANG Ting; CUI Yinhang; WU Peibin; CHEN Wenlong; CHEN Huilin; LIN Caizhi; TANG Meiwen

doi:10.6039/j.issn.1001-0408.2025.21.07

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Study on the inhibitory effect and mechanism of Modified qifang weitong granules on gastric cancer

更新时间：2025-11-10

- Study on the inhibitory effect and mechanism of Modified qifang weitong granules on gastric cancer
- ZHONGGUO YAOFANG Vol. 36, Issue 21, Pages: 2656-2661(2025)
- 作者机构：
  
  1.广西中医药大学研究生院，南宁　530000
  2.广西中医药大学第一附属医院脾胃肝病科，南宁　530219
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2025.21.07
  CLC： R965
- Received：13 May 2025，
  
  Revised：2025-10-05，
  
  Accepted：09 October 2025，
  
  Published：15 November 2025
- 稿件说明：
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陈鑫源,吴成挺,熊常州,等.加味七方胃痛颗粒对胃癌的抑制作用及机制研究[J].中国药房,2025,36(21):2656-2661.

CHEN Xinyuan,WU Chengting,XIONG Changzhou,et al.Study on the inhibitory effect and mechanism of Modified qifang weitong granules on gastric cancer[J].ZHONGGUO YAOFANG,2025,36(21):2656-2661.
陈鑫源,吴成挺,熊常州,等.加味七方胃痛颗粒对胃癌的抑制作用及机制研究[J].中国药房,2025,36(21):2656-2661. DOI： 10.6039/j.issn.1001-0408.2025.21.07.

CHEN Xinyuan,WU Chengting,XIONG Changzhou,et al.Study on the inhibitory effect and mechanism of Modified qifang weitong granules on gastric cancer[J].ZHONGGUO YAOFANG,2025,36(21):2656-2661. DOI： 10.6039/j.issn.1001-0408.2025.21.07.

摘要

目的

结合体内外实验研究加味七方胃痛颗粒对胃癌的抑制作用及机制。

方法

将人胃癌HGC-27细胞分为对照组（胎牛血清）、10%含药血清组、15%含药血清组、20%含药血清组、5-氟尿嘧啶（5-Fu）组（阳性对照，3.90 μg/mL），各组细胞给予相应血清/药液培养后，检测其增殖、迁移、侵袭能力以及细胞周期，检测细胞中上皮-间充质转化（EMT）相关蛋白[E-钙黏蛋白（E-cadherin）、N-钙黏蛋白（N-cadherin）、波形蛋白（vimentin）]表达水平。另取对数期HGC-27细胞，经皮下注射至裸鼠右侧腋下，以建立裸鼠皮下移植瘤模型。将造模成功的荷瘤裸鼠随机分为模型组和加味七方胃痛颗粒低、中、高剂量组（17.65、35.29、70.58 g/kg）以及5-Fu组（25 mg/kg），每组5只。各组裸鼠给予相应药物处理14 d后，观察裸鼠肿瘤组织病理学形态，采用免疫组化法和Western blot法检测裸鼠肿瘤组织中EMT相关蛋白表达水平。

结果

细胞实验中，与对照组相比，15%含药血清组、20%含药血清组的细胞增殖率、迁移率、侵袭数和N-cadherin、vimentin蛋白表达水平以及G

/M期细胞百分比均显著降低/减少（

＜0.05），G

期细胞百分比和E-cadherin蛋白表达水平均显著升高（

＜0.05）。动物实验中，与模型组相比，加味七方胃痛颗粒高剂量组裸鼠的肿瘤质量和肿瘤组织中N-cadherin、vimentin蛋白表达水平均显著降低（

＜0.05），肿瘤组织中E-cadherin蛋白表达水平显著升高（

＜0.05）；肿瘤细胞大小不一，且大量坏死。

结论

加味七方胃痛颗粒在体内外模型中均能有效抑制胃癌，其作用机制与抑制EMT有关。

Abstract

OBJECTIVE

To investigate the inhibitory effect and mechanism of Modified qifang weitong granules on gastric cancer based on

in vitro

and

in vivo

experiments.

METHODS

Human gastric cancer HGC-27 cells were divided into the following groups: control group （treated with fetal bovine serum）， 10% drug-containing serum group， 15% drug-containing serum group， 20% drug-containing serum group， and 5-fluorouracil （5-Fu） group （positive control， 3.90 μg/mL）. After culturing the cells in each group with the corresponding serum/drug solution， their proliferation， migratory and invasive abilities， as well as the cell cycle， were assessed. Additionally， the expression levels of epithelial-mesenchymal transition （EMT）-related proteins [E-cadherin， N-cadherin， and vimentin] in the cells were measured. Logarithmic-phase HGC-27 cells were harvested and subcutaneously injected into the right axillary region of nude mice to establish a subcutaneous xenograft tumor model in nude mice. The successfully modeled tumor-bearing nude mice were randomly divided into model group， low-， medium- and high-dose groups of Modified qifang weitong granules （17.65， 35.29 and 70.58 g/kg， respectively）， and 5-Fu group （25 mg/kg）， with 5 mice in each group. After 14 days of treatment with the corresponding drugs in each group， the histopathological morphology of the tumor tissues in the nude mice was observed. Immunohistochemistry and Western blot assay were employed to detect the expression levels of EMT-related proteins in the tumor tissues of the nude mice.

RESULTS

In the cell experiment， compared with the control group， the cell proliferation rate， migration rate， number of invasive cells， as well as the expression levels of N-cadherin and vimentin proteins， and the percentage of cells in the G

/M phase were all significantly decreased/reduced in the 15% drug-containing serum group， 20% drug-containing serum group （

＜0.05）.

Conversely， the percentage of cells in the G

phase and the expression level of E-cadherin protein were significantly increased （

＜0.05）. In animal experiment， compared with the model group， the high-dose group of Modified qifang weitong granules exhibited significantly reduced tumor mass and expression levels of N-cadherin and vimentin proteins in the tumor tissues of nude mice （

＜0.05）， while the expression level of E-cadherin protein in the tumor tissues was significantly increased （

＜0.05）. Additionally， the tumor cells varied in size and showed extensive necrosis.

CONCLUSIONS

Modified qifang weitong granules effectively inhibit gastric cancer in both

in vitro

and

in vivo

models， and the mechanism of action is related to the suppression of EMT.

关键词

Keywords

references

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