OBJECTIVE

2

To improve the quality standard of Zhuang medicine Yingbupu （

Aralia armata

）.

METHODS

2

A total of 23 batches of Yingbupu （

A. armata

） were studied. Their macroscopic characteristics and powder microscopic features were observed. TLC was employed for the qualitative identification of oleanolic acid and araloside A. Items such as water content， total ash， acid-insoluble ash， and ethanol-soluble extract were determined according to the methods specified in the 2020 edition of the

Chinese Pharmacopoeia

（part Ⅳ）. UPLC fingerprint was established for 23 batches of samples by using

Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine

（

2012 edition

）， and the contents of oleanolic acid and araloside A were determined.

RESULTS

2

The powder microscopic characteristics of the medicinal material were distinctive. Oleanolic acid and araloside A were detected by TLC in all 23 batches. Among the 23 batches of samples， the content ranges of moisture， total ash， acid-insoluble ash， and ethanol-soluble extract were 6.9% to 10.4%， 1.8% to 6.8%， 0.1% to 1.9%， and 2.8% to 8.4%， respectively. Based on the UPLC fingerprint， a total of 15 common peaks were obtained， and 9 of these common peaks were identified. The content ranges of oleanolic acid and araloside A in the 23 batches of samples were 0.86% to 2.69% and 0.16% to 1.10%， respectively.

CONCLUSIONS

2

This study has added items such as moisture and total ash content fingerprint， TLC identification. A preliminary quality standard has been established for the medicinal material of Yingbupu （

A. armata

）， stipulating that the moisture content should not exceed 11.0%， the total ash content should not exceed 5.0%， the acid-insoluble ash content should not exceed 2.5%， the ethanol-soluble extract content should not be less than 4.0%， and the contents of oleanolic acid and araloside A should not be less than 1.00% and 0.45% （calculated by a dried basis）， respectively.