Study on the mechanism of <italic style="font-style: italic">Lycium barbarum</italic> polysaccharides inhibiting the proliferation， migration and immune escape of oral cancer cells

LI Jinyu; XU Xiaoyu; WANG Yuzhuo

doi:10.6039/j.issn.1001-0408.2025.17.09

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Study on the mechanism of Lycium barbarum polysaccharides inhibiting the proliferation， migration and immune escape of oral cancer cells

更新时间：2025-09-29

- Study on the mechanism of Lycium barbarum polysaccharides inhibiting the proliferation， migration and immune escape of oral cancer cells
- ZHONGGUO YAOFANG Vol. 36, Issue 17, Pages: 2134-2140(2025)
- 作者机构：
  
  安徽医科大学附属滁州医院/滁州市第一人民医院口腔科，安徽滁州　239000
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2025.17.09
  CLC： R965
- Received：27 May 2025，
  
  Revised：2025-07-28，
  
  Accepted：28 July 2025，
  
  Published：15 September 2025
- 稿件说明：
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李锦宇,徐晓雨,王钰卓.枸杞多糖抑制口腔癌细胞增殖、迁移及免疫逃逸的机制研究[J].中国药房,2025,36(17):2134-2140.

LI Jinyu,XU Xiaoyu,WANG Yuzhuo.Study on the mechanism of Lycium barbarum polysaccharides inhibiting the proliferation， migration and immune escape of oral cancer cells[J].ZHONGGUO YAOFANG,2025,36(17):2134-2140.
李锦宇,徐晓雨,王钰卓.枸杞多糖抑制口腔癌细胞增殖、迁移及免疫逃逸的机制研究[J].中国药房,2025,36(17):2134-2140. DOI： 10.6039/j.issn.1001-0408.2025.17.09.

LI Jinyu,XU Xiaoyu,WANG Yuzhuo.Study on the mechanism of Lycium barbarum polysaccharides inhibiting the proliferation， migration and immune escape of oral cancer cells[J].ZHONGGUO YAOFANG,2025,36(17):2134-2140. DOI： 10.6039/j.issn.1001-0408.2025.17.09.

摘要

目的

探索枸杞多糖调节T细胞免疫球蛋白黏蛋白3（TIM3）/半乳糖凝集素9（Gal-9）信号通路对口腔癌细胞增殖、迁移和免疫逃逸的影响。

方法

将人口腔癌KB、CAL27细胞分为对照组、低浓度枸杞多糖组（200 μg/mL）、高浓度枸杞多糖组（400 μg/mL）、pcDNA-NC组（转染pcDNA-NC质粒）、pcDNA-TIM3组（转染pcDNA-TIM3质粒）、高浓度枸杞多糖+pcDNA-NC组（400 μg/mL枸杞多糖+转染pcDNA-NC质粒）、高浓度枸杞多糖+pcDNA-TIM3组（400 μg/mL枸杞多糖+转染pcDNA-TIM3质粒）。测定细胞的增殖、迁移、侵袭能力，T细胞杀伤率，细胞上清液中干扰素γ（IFN-γ）、白细胞介素2（IL-2）水平，以及细胞中TIM3、Gal-9 mRNA表达，以及细胞中吲哚胺2，3-双加氧酶1（IDO1）、程序性死亡受体配体1（PD-L1）、TIM3、Gal-9蛋白表达。

结果

与对照组比较，低、高浓度枸杞多糖组2种细胞的克隆形成率、划痕愈合率、侵袭细胞数以及IDO1、PD-L1蛋白和TIM3、Gal-9 mRNA及其蛋白表达水平均显著降低/减少（

＜0.05），增殖抑制率、T细胞杀伤率和IFN-γ、IL-2水平均显著升高（

＜0.05）；与对照组、pcDNA-NC组比较，pcDNA-TIM3组2种细胞的克隆形成率、划痕愈合率、侵袭细胞数以及IDO1、PD-L1蛋白和TIM3、Gal-9 mRNA及其蛋白表达水平均显著升高/增加（

＜0.05），增殖抑制率、T细胞杀伤率和IFN-γ、IL-2水平均显著降低（

＜0.05）；与高浓度枸杞多糖组、高浓度枸杞多糖+pcDNA-NC组比较，高浓度枸杞多糖+pcDNA-TIM3组2种细胞的克隆形成率、划痕愈合率、侵袭细胞数以及IDO1、PD-L1蛋白和TIM3、Gal-9 mRNA及其蛋白表达水平均显著升高/增加（

＜0.05），增殖抑制率、T细胞杀伤率和IFN-γ、IL-2水平均显著降低（

＜0.05）。

结论

枸杞多糖可能通过抑制TIM3/Gal-9信号通路来抑制口腔癌细胞增殖、迁移和免疫逃逸。

Abstract

OBJECTIVE

To explore the effects of

Lycium barbarum

polysaccharides on the proliferation， migration， and immune escape of oral cancer cells by regulating the T cell immunoglobulin and mucin domain-containing protein 3 （TIM3）/galectin-9 （Gal-9） signaling pathway.

METHODS

Human oral cancer cells KB and CAL27 were assigned to control group，

L. barbarum

polysaccharides low-concentration group （200 μg/mL），

L. barbarum

polysaccharides high-concentration gr

oup （400 μg/mL）， pcDNA-NC group （transfection of pcDNA-NC plasmid）， pcDNA-TIM3 group （transfection of pcDNA-TIM3 plasmid）， high concentration of

L. barbarum

polysaccharides+pcDNA-NC group （400 μg/mL

L. barbarum

polysaccharides + transfection of pcDNA-NC plasmid）， and high concentration of

L. barbarum

polysaccharides+pcDNA-TIM3 group （400 μg/mL

L. barbarum

polysaccharides + transfection of pcDNA-TIM3 plasmid）. The proliferation， migration and invasion abilities of the cells， T cell killing rate as well as the levels of interferon-γ （IFN-γ） and interleukin-2 （IL-2） in the cell supernatant were measured. mRNA expressions of TIM3 and Gal-9 and protein expressions of indoleamine 2，3-dioxygenase 1 （IDO1）， programmed death-ligand 1 （PD-L1）， TIM3 and Gal-9 in the cells were also determined.

RESULTS

Compared with control group， the clone formation rate， scratch healing rate， the number of invasive cells， protein expressions of IDO1 and PD-L1， mRNA and protein expressions of TIM3 and Gal-9 in both cell types of

L. barbarum

polysaccharide low- and high-concentration groups were decreased significantly （

＜0.05）， while the proliferation inhibition rate， T cell killing rate， and the levels of IFN-γ and IL-2 were significantly increased （

＜0.05）. Compared with control group and pcDNA-NC group， the clone formation rate， scratch healing rate， the number of invasive cells， and protein expressions of IDO1 and PD-L1， mRNA and protein expressions of TIM3 and Gal-9 in both cell types of the pcDNA-TIM3 group were all significantly increased （

＜0.05）， while the proliferation inhibition rate， T cell killing rate， IFN-γ and IL-2 levels were significantly decreased （

＜0.05）. Compared with

L. barbarum

polysaccharides high-concentration group and high concentration of

L. barbarum

polysaccharides+pcDNA-NC group， the clone fo

rmation rate， scratch healing rate， the number of invasive cells， and protein expressions of IDO1 and PD-L1， mRNA and protein expressions of TIM3 and Gal-9 in both cell types of high concentration of

L. barbarum

polysaccharides+pcDNA-TIM3 group were significantly increased （

＜0.05）， while the proliferation inhibition rate， T cell killing rate， and the levels of IFN-γ and IL-2 were significantly decreased （

＜0.05）.

CONCLUSIONS

L. barbarum

polysaccharides may inhibit the proliferation， migration， and immune escape of oral cancer cells by suppressing TIM3/Gal-9 signaling pathway.

关键词

Keywords

references

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Study on the mechanism of Lycium barbarum polysaccharides inhibiting the proliferation， migration and immune escape of oral cancer cells

DOI：10.6039/j.issn.1001-0408.2025.17.09

摘要

Abstract

关键词

Keywords

references