Effect and mechanism of paeonol in regulating NF-κB/HIF-1α signaling pathway to inhibit the migration of bladder cancer T24 cells

AI Xinyao; CHEN Wenjia; CHEN Xi; WANG Yingzheng; WANG Yinghao; HUANG Meixia

doi:10.6039/j.issn.1001-0408.2025.15.09

您当前的位置：

首页 >

文章列表页 >

Effect and mechanism of paeonol in regulating NF-κB/HIF-1α signaling pathway to inhibit the migration of bladder cancer T24 cells

更新时间：2025-11-14

- Effect and mechanism of paeonol in regulating NF-κB/HIF-1α signaling pathway to inhibit the migration of bladder cancer T24 cells
- ZHONGGUO YAOFANG Vol. 36, Issue 15, Pages: 1871-1875(2025)
- 作者机构：
  
  福建中医药大学药学院，福州　350122
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2025.15.09
  CLC： R965
- Received：28 April 2025，
  
  Revised：2025-05-18，
  
  Accepted：04 July 2025，
  
  Published：15 August 2025
- 稿件说明：
移动端阅览
艾欣瑶,陈雯佳,陈曦,等.丹皮酚调控NF-κB/HIF-1α信号通路抑制膀胱癌T24细胞迁移的作用及机制[J].中国药房,2025,36(15):1871-1875.

AI Xinyao,CHEN Wenjia,CHEN Xi,et al.Effect and mechanism of paeonol in regulating NF-κB/HIF-1α signaling pathway to inhibit the migration of bladder cancer T24 cells[J].ZHONGGUO YAOFANG,2025,36(15):1871-1875.
艾欣瑶,陈雯佳,陈曦,等.丹皮酚调控NF-κB/HIF-1α信号通路抑制膀胱癌T24细胞迁移的作用及机制[J].中国药房,2025,36(15):1871-1875. DOI： 10.6039/j.issn.1001-0408.2025.15.09.

AI Xinyao,CHEN Wenjia,CHEN Xi,et al.Effect and mechanism of paeonol in regulating NF-κB/HIF-1α signaling pathway to inhibit the migration of bladder cancer T24 cells[J].ZHONGGUO YAOFANG,2025,36(15):1871-1875. DOI： 10.6039/j.issn.1001-0408.2025.15.09.

摘要

目的

探讨丹皮酚调控核因子κB（NF-κB）/缺氧诱导因子1α（HIF-1α）信号通路介导的有氧糖酵解从而抑制膀胱癌T24细胞迁移的作用及机制。

方法

将T24细胞分为对照组、顺铂组（阳性对照，3.001 μg/mL）和丹皮酚低、中、高剂量组（100、200、400 μg/mL），给药干预24 h后，检测丹皮酚对T24细胞迁移能力的影响（以细胞划痕愈合率表示），检测丹皮酚对T24细胞线粒体膜电位的影响（以红绿荧光强度比值表示），检测T24细胞中腺苷三磷酸（ATP）浓度和乳酸含量，检测细胞中NF-κB/HIF-1α信号通路、迁移相关蛋白以及有氧糖酵解关键酶的表达水平。

结果

与对照组比较，丹皮酚中、高剂量组和顺铂组细胞划痕愈合率均显著降低（

＜0.01）；丹皮酚各剂量组细胞中NF-κB/HIF-1α信号通路相关蛋白NF-κB、HIF-1α，迁移相关蛋白基质金属蛋白酶2（MMP2）、MMP9、血管内皮生长因子，有氧糖酵解关键酶葡萄糖转运蛋白1、己糖激酶2、丙酮酸激酶M2表达水平均有不同程度降低，大部分差异有统计学意义（

＜0.05或

＜0.01）；丹皮酚中、高剂量组细胞线粒体红绿荧光强度比值均显著降低（

＜0.01）；丹皮酚高剂量组细胞ATP浓度以及丹皮酚各剂量组细胞乳酸含量均显著降低（

＜0.05或

＜0.01）。

结论

丹皮酚可抑制膀胱癌T24细胞的迁移，其作用机制可能与抑制NF-κB/HIF-1α信号通路、下调有氧糖酵解关键酶活性有关。

Abstract

OBJECTIVE

To investigate the role and mechanism of paeonol in inhibiting the migration of bladder cancer T24 cells by regulating nuclear factor κB （NF-κB）/hypoxia-inducible factor-1α （HIF-1α）-mediated aerobic glycolysis.

METHODS

T24 cells were divided into control group， cisplatin group （positive control， 3.001 μg/mL）， and paeonol low-， medium- and high-dose groups （100， 200， 400 μg/mL）， respectively. After 24 h of administration intervention， the effect of paeonol on the migration ability of T24 cells was detected （expressed by the cell scratch wound healing rate）. The effect of paeonol on the mitochondrial membrane potential of T24 cells was detected （expressed by the ratio of red/green fluorescence intensity）. Cellular adenosine triphosphate （ATP） levels and lactate content in T24 cells were measured. The levels of NF-κB/HIF-1α signaling pathway， the expression of migration-related proteins， and key enzymes involved in aerobic glycolysis in the cells were all determined.

RESULTS

Compared with the control group， the cell scratch wound healing rates in the paeonol medium- and high-dose groups and the cisplatin group were decreased significantly （

＜0.01）； in the paeonol groups， the expression levels of NF-κB/HIF-1α signaling pathway-related proteins such as NF-κB and HIF-1α， migration-related proteins such as matrix metalloproteinase 2 （MMP2）， MMP9， and vascular endothelial growth factor， as well as key enzymes involved in aerobic glycolysis such as glucose transporter 1， hexokinase 2 and pyruvate kinase isozyme type M2， were all reduced to varying degrees in the cells， most of these reductions showed statistically significant differences （

＜0.05 or

＜0.01）； the ratio of red/green fluorescence intensity in mitochondria of cells in the medium- and high-dose paeonol groups

were significantly decreased （

＜0.01）； the ATP concentration in cells of the paeonol high-dose group， and the lactate content in cells across all paeonol groups were significantly decreased （

＜0.05 or

＜0.01）.

CONCLUSIONS

Paeonol significantly inhibits the migration of bladder cancer T24 cells， and its mechanism of action may be related to the inhibition of the NF-κB/HIF-1α signaling pathway， and the down-regulation of key enzyme activities involved in aerobic glycolysis.

关键词

Keywords

references

MIRTO B F ， BARONE B ， BALSAMO R ， et al . Early and late post-procedural complications in different orthotopic neobladder surgical approaches：a systematic review [J ] . Surg Oncol ， 2024 ， 55 ： 102090 .

LIU H ， WANG S ， WANG J H ， et al . Energy metabolism in health and diseases [J ] . Signal Transduct Target Ther ， 2025 ， 10 （ 1 ）： 69 .

ANDRYSZKIEWICZ W ， GĄSIOROWSKA J ， KÜBLER M ， et al . Glucose metabolism and tumor microenvironment：mechanistic insights and therapeutic implications [J ] . Int J Mol Sci ， 2025 ， 26 （ 5 ）： 1879 .

BAO X T ， ZHANG J H ， HUANG G M ， et al . The crosstalk between HIFs and mitochondrial dysfunctions in cancer development [J ] . Cell Death Dis ， 2021 ， 12 （ 2 ）： 215 .

YANG H L ， CHANG C W ， VADIVALAGAN C ， et al . Coenzyme Q 0 inhibited the NLRP3 inflammasome，metastasis/EMT，and Warburg effect by suppressing hypoxia-induced HIF-1α expression in HNSCC cells [J ] . Int J Biol Sci ， 2024 ， 20 （ 8 ）： 2790 - 2813 .

GUO J ， MENG F Q ， HU R X ， et al . Inhibition of the NF-κB/HIF-1α signaling pathway in colorectal cancer by tyrosol：a gut microbiota-derived metabolite [J ] . J Immunother Cancer ， 2024 ， 12 （ 9 ）： e008831 .

QING L L ， LI Q C ， YANG Y J ， et al . Hypoxia-mediated attenuation of EGLN2 inhibition of the NF-κB signaling pathway leads to the formation of a loop between HIF-1α and MUC1-C promoting chemoresistance in bladder cancer [J ] . Mol Carcinog ， 2024 ， 63 （ 7 ）： 1303 - 1318 .

YANG S J ， LI L J . Pharmacological effects and application of paeonol [J ] . Pharmacol Clin Chin Mater Med ， 2022 ， 38 （ 5 ）： 237 - 241 .

CHEN G ， JIA P ， YIN Z Y ， et al . Paeonol ameliorates monosodium urate-induced arthritis in rats through inhibi- ting nuclear factor-κB-mediated proinflammatory cytokine production [J ] . Phytother Res ， 2019 ， 33 （ 11 ）： 2971 - 2978 .

PANG C H ， WU Z J ， XU X Y ， et al . Paeonol alleviates migration and invasion of endometrial stromal cells by reducing HIF-1α-regulated autophagy in endometriosis [J ] . Front Biosci （Landmark Ed）， 2021 ， 26 （ 9 ）： 485 - 495 .

袁乡石，苏羽屾，荣冬芸，等 . 松油烯-4-醇通过线粒体途径诱导宫颈癌Siha细胞凋亡的机制研究 [J ] . 中成药， 2023 ， 45 （ 10 ）： 3433 - 3438 .

ANTONI S ， FERLAY J ， SOERJOMATARAM I ， et al . Bladder cancer incidence and mortality：a global overview and recent trends [J ] . Eur Urol ， 2017 ， 71 （ 1 ）： 96 - 108 .

WANG Y J ， LI Z J ， TENG M Z ， et al . Dihydroartemisinin inhibits activation of the AIM2 inflammasome pathway and NF-κB/HIF-1α/VEGF pathway by inducing autophagy in A431 human cutaneous squamous cell carcinoma cells [J ] . Int J Med Sci ， 2021 ， 18 （ 12 ）： 2705 - 2715 .

张维，胡晟 . 阿帕替尼调节VEGF/MAPK/NF-κB信号通路抑制大鼠非小细胞肺癌的机制 [J ] . 生物技术， 2022 ， 32 （ 2 ）： 195 - 199 .

NING X ， WANG Y ， YAN W ， et al . Chitin synthesis inhibitors promote liver cancer cell metastasis via interfering with hypoxia-inducible factor 1α [J ] . Chemosphere ， 2018 ， 206 ： 231 - 237 .

KAI A K ， CHAN L K ， LO R C ， et al . Down-regulation of TIMP2 by HIF-1α/miR-210/HIF-3α regulatory feedback circuit enhances cancer metastasis in hepatocellular carcinoma [J ] . Hepatology ， 2016 ， 64 （ 2 ）： 473 - 487 .

D’IGNAZIO L ， BATIE M ， ROCHA S . Hypoxia and inflammation in cancer，focus on HIF and NF-κB [J ] . Biomedicines ， 2017 ， 5 （ 2 ）： 21 .

VAUPEL P ， SCHMIDBERGER H ， MAYER A . The Warburg effect：essential part of metabolic reprogramming and central contributor to cancer progression [J ] . Int J Radiat Biol ， 2019 ， 95 （ 7 ）： 912 - 919 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect and mechanism of baicalin on the proliferation and migration of human periodontal ligament stem cells induced by lipopolysaccharide

Inhibitory effects of ursolic acid on IL-6-mediated invasion and migration of breast cancer cells

木犀草素对肝星状细胞迁移和增殖的影响Δ

利巴韦林对人肺腺癌细胞A549、人乳腺癌细胞Hela迁移和凋亡的影响研究

紫杉醇联合顺铂对甲状腺癌细胞SW579增殖、迁移和侵袭作用的影响

Related Author

WANG Qiao

GAO Yongzhi

FENG Bo

XIANG Fenfen

ZHANG Tao

CHEN Zixi

ZHANG Mengzhe

KANG Xiangdong

Related Institution

Dept. of Stomatology， the Second Affiliated Hospital of Qiqihar Medical College

Dept. of Stomatology， Qiqihar Wuguan Hospital

Dept. of Stomatology， the First Affiliated Hospital of Qiqihar Medical College

Dept. of Laboratory， Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

⁰