Establishment of quantitative analysis of multi-components by single-marker method for content determination of flavonoids and phenolic acids in <italic style="font-style: italic">Perilla frutescens</italic> leaves

LI Danyang; DONG Chao; ZHENG Yunfeng; YAN Hui; ZHANG Li

doi:10.6039/j.issn.1001-0408.2025.11.06

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Establishment of quantitative analysis of multi-components by single-marker method for content determination of flavonoids and phenolic acids in Perilla frutescens leaves

更新时间：2026-02-06

- Establishment of quantitative analysis of multi-components by single-marker method for content determination of flavonoids and phenolic acids in Perilla frutescens leaves
- ZHONGGUO YAOFANG Vol. 36, Issue 11, Pages: 1323-1328(2025)
- 作者机构：
  
  1.江苏省中医院药学部，南京　210029
  2.南京医科大学第一附属医院药学部，南京　210029
  3.南京中医药大学药学院，南京　210023
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2025.11.06
  CLC： R917;R284.1
- Received：15 December 2024，
  
  Revised：2025-05-19，
  
  Accepted：20 May 2025，
  
  Published：15 June 2025
- 稿件说明：
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李丹阳,董超,郑云枫,等.紫苏叶中黄酮及酚酸类成分一测多评含量测定方法的建立 [J].中国药房,2025,36(11):1323-1328.

LI Danyang,DONG Chao,ZHENG Yunfeng,et al.Establishment of quantitative analysis of multi-components by single-marker method for content determination of flavonoids and phenolic acids in Perilla frutescens leaves[J].ZHONGGUO YAOFANG,2025,36(11):1323-1328.
李丹阳,董超,郑云枫,等.紫苏叶中黄酮及酚酸类成分一测多评含量测定方法的建立 [J].中国药房,2025,36(11):1323-1328. DOI： 10.6039/j.issn.1001-0408.2025.11.06.

LI Danyang,DONG Chao,ZHENG Yunfeng,et al.Establishment of quantitative analysis of multi-components by single-marker method for content determination of flavonoids and phenolic acids in Perilla frutescens leaves[J].ZHONGGUO YAOFANG,2025,36(11):1323-1328. DOI： 10.6039/j.issn.1001-0408.2025.11.06.

摘要

目的

以野黄芩苷、迷迭香酸为内参物，建立同时测定紫苏叶中6种黄酮类及2种酚酸类成分含量的一测多评（QAMS）法，并采用该法测定20批不同产地紫苏叶样品中8种成分的含量。

方法

以野黄芩苷为内参物计算野黄芩素-7-

-二葡萄糖醛酸苷、木犀草素-7-

-二葡

萄糖醛酸苷、芹菜素-7-

-二葡萄糖醛酸苷、木犀草素-7-

-D-葡萄糖醛酸苷、芹菜素-7-

-葡萄糖醛酸苷的相对校正因子，以迷迭香酸为内参物计算咖啡酸的相对校正因子，采用QAMS法测定样品中各成分的含量，并与外标法检测结果进行比较。

结果

上述8种成分在各自质量浓度范围内线性关系良好（

≥0.999 0），平均加样回收率为95.60%～102.15%，RSD为0.72%～2.70%（

＝6）。该方法的精密度、重复性、稳定性均良好（RSD＜2.50%，

＝6）。不同仪器和色谱柱、柱温、流速、甲酸体积分数对所得相对校正因子的影响较小（RSD＜3%，

＝3）。与外标法比较，除ZS12批次样品中的咖啡酸外，其余各批次样品中各成分的含量均无明显差异（RE绝对值＜5%，

＝2）。不同产地紫苏叶样品中6种黄酮类成分的含量存在一定差异，而上述黄酮类成分的总含量无明显差异；不同产地紫苏叶中2种酚酸类成分的含量及其总含量具有明显差异。

结论

所建QAMS法可同时测定紫苏叶药材中6种黄酮类及2种酚酸类成分的含量，方法检测方便、准确度高且成本较低，可用于紫苏叶药材的质量控制。不同产地紫苏叶药材中黄酮类及酚酸类成分的含量存在一定差异，可为紫苏叶药材产地的优选提供参考依据。

Abstract

OBJECTIVE

To establish a quantitative analysis of multi-components by single-marker （QAMS） method for simultaneous determination of six flavonoids and two phenolic acids in

Perilla frutescens

leaves using scutellarin and rosmarinic acid as internal reference substances， and apply this method to determine the contents of eight components in 20 batches of

P. frutescens

leaves samples from different regions.

METHODS

Scutellarin served as the internal reference to calculate relative correction factors （RCFs） for scutellarin-7-

-diglucuronide， luteolin-7-

-diglucuronide， apigenin-7-

-diglucuronide， luteolin-7-

-D-glucuronide and apigenin-7-

-glucuronide. Rosmarinic acid was employed as the internal reference to determine the RCF for caffeic acid. The contents of the above flavonoids and phenolic acids were calculated with QAMS， and compared with the results of external standard method.

RESULTS

The eight analytes demonstrated excellent linearity within their respective concentration ranges

（

≥0.999 0）. The mean recovery rates for spiked samples ranged from 95.60% to 102.15%， with relative standard deviations （RSDs） of 0.72% to 2.70% （

＝6）. The method exhibited good precision， repeatability， and stability （RSD＜2.50%，

＝6）. Variations in instruments， columns， column temperature， flow rate， and formic acid volume fraction had minimal impact on the RCFs （RSD＜3%，

＝3）. Comparison with the external standard method showed no significant differences in the content of each component across batches， except for caffeic acid in the ZS12 batch （absolute value of RE＜5%，

＝2）. The contents of six flavonoid components in

P. frutescens

leaves samples varied significantly across different geographic origins， while the content of total flavonoids showed no significant difference. In contrast， the contents of two phenolic acid components and total phenolic acid exhibited significant variation among samples from different regions.

CONCLUSIONS

The developed QAMS method can simultaneously determine the contents of six flavonoids and two phenolic acids in

P. frutescens

leaves. It is convenient for detection， highly accurate， and cost-effective. This method is suitable for the quality control of

P. frutescens

leaves， and the variation of flavonoid and phenolic acid content in samples from different regions provides a reference for the selection of optimal cultivation areas.

关键词

Keywords

references

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Establishment of quantitative analysis of multi-components by single-marker method for content determination of flavonoids and phenolic acids in Perilla frutescens leaves

DOI：10.6039/j.issn.1001-0408.2025.11.06

摘要

Abstract

关键词

Keywords

references