Fundamental study on the anti-inflammatory activity of the petroleum ether extract from <italic style="font-style: italic">Melastoma dodecandrum</italic> based on spectrum-effect relationship

LUO Shixi; MA Xiuping; LIU Jing; XIONG Wuqing; SUN Qingwen; DING Ning

doi:10.6039/j.issn.1001-0408.2024.23.07

您当前的位置：

首页 >

文章列表页 >

Fundamental study on the anti-inflammatory activity of the petroleum ether extract from Melastoma dodecandrum based on spectrum-effect relationship

更新时间：2025-09-25

- Fundamental study on the anti-inflammatory activity of the petroleum ether extract from Melastoma dodecandrum based on spectrum-effect relationship
- ZHONGGUO YAOFANG Vol. 35, Issue 23, Pages: 2877-2882(2024)
- 作者机构：
  
  1.贵州中医药大学药学院，贵阳　550025
  2.国家苗药工程技术研究中心/贵州中药炮制与制剂工程技术研究中心，贵阳　550025
  3.贵州中医药大学茶+大健康食品开发研究中心，贵阳　550025
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.23.07
  CLC： R917;R284
- Received：17 June 2024，
  
  Revised：2024-09-18，
  
  Accepted：19 September 2024，
  
  Published：15 December 2024
- 稿件说明：
移动端阅览
罗仕西,麻秀萍,刘静,等.基于谱效关系的地菍石油醚提取物抗炎活性物质基础研究[J].中国药房,2024,35(23):2877-2882.

LUO Shixi,MA Xiuping,LIU Jing,et al.Fundamental study on the anti-inflammatory activity of the petroleum ether extract from Melastoma dodecandrum based on spectrum-effect relationship[J].ZHONGGUO YAOFANG,2024,35(23):2877-2882.
罗仕西,麻秀萍,刘静,等.基于谱效关系的地菍石油醚提取物抗炎活性物质基础研究[J].中国药房,2024,35(23):2877-2882. DOI： 10.6039/j.issn.1001-0408.2024.23.07.

LUO Shixi,MA Xiuping,LIU Jing,et al.Fundamental study on the anti-inflammatory activity of the petroleum ether extract from Melastoma dodecandrum based on spectrum-effect relationship[J].ZHONGGUO YAOFANG,2024,35(23):2877-2882. DOI： 10.6039/j.issn.1001-0408.2024.23.07.

摘要

目的

建立地菍石油醚提取物的指纹图谱，并结合细胞药效学实验探究其抗炎活性物质基础。

方法

采用高效液相色谱（HPLC）法，利用《中药色谱指纹图谱相似度评价系统（2012版）》生成20批地菍石油醚提取物的指纹图谱，并进行相似度评价和共有峰指认；建立脂多糖诱导的小鼠单核巨噬细胞RAW264.7炎症模型，以一氧化氮（NO）、肿瘤坏死因子α（TNF-α）的抑制率为指标，考察地菍石油醚提取物的抗炎活性，并运用灰色关联度法、偏最小二乘回归分析法进行谱效关系研究；采用分子对接验证抗炎活性成分与TNF-α、诱导型一氧化氮合酶（iNOS）蛋白质受体的结合活性。

结果

20批地菍石油醚提取物的指纹图谱中有19个共有峰，20批样品的相似度为0.603～0.990；指认了牡荆素（5号峰）、异牡荆素（6号峰）、鞣花酸（7号峰）、槲皮素（9号峰）、木犀草素（10号峰）5个成分。地菍石油醚提取物19个共有峰与NO、TNF-α抑制率的关联度均大于0.7，峰19、13、9（槲皮素）、12、5（牡荆素）、6（异牡荆素）、8、7（鞣花酸）、18、1与NO抑制率呈正相关；峰8、10（木犀草素）、13、15、3、19、17、7（鞣花酸）、18、1与TNF-α抑制率呈正相关。牡荆素、异牡荆素、槲皮素与iNOS蛋白质受体的结合能均小于－5.0 kcal/mol。

结论

牡荆素、异牡荆素、槲皮素可能是地菍石油醚提取物发挥抗炎作用的药效物质基础。

Abstract

OBJECTIVE

To explore the material basis of the anti-inflammatory effect of the petroleum ether extract from

Melastoma dodecandrum

by establishing its fingerprint and combining it with cellular pharmacodynamics experiments.

METHODS

HPLC method was adopted； the fingerprints of 20 batches of petroleum ether extract from

M. dodecandrum

were drawn using

The Similarity Evaluation System of TCM Chromatographic Fingerprint

（

2012 edition

）； similarity evaluation and common peak identification were carried out. The lipopolysaccharide-induced inflammation model of mice mononuclear macrophages （RAW264.7） was established； the inhibitory rates of nitric oxide （NO） and tumor necrosis factor α （TNF-α） were used as indexes to investigate the anti-inflammatory activity of the petroleum ether extract from

M. dodecandrum

； grey correlation degree method and partial least square regression analysis were adopted to study the spectrum-effect relationship. Molecular docking was used to validate the binding activity of the anti-inflammatory active ingredients with TNF-α and iNOS protein receptor.

RESULTS

There were 19 common peaks in the fingerprint of the petroleum ether extract from

M. dodecandrum

， the similarity of 20 batches of samples ranged from 0.603-0.990， and five components were identified， such as vitexin （peak 5）， isovitexin （peak 6）， ellagic acid （peak 7）， quercetin （peak 9） and luteolin （peak 10）. The grey correlation degree between 19 common peaks of the petroleum ether extract from

M. dodecandrum

and the inhibition rates of NO and TNF-α were all greater than 0.7； peaks 19， 13， 9 （quercetin）， 12， 5 （vitexin）， 6 （isovitexin）， 8， 7 （ellagic acid）， 18， 1 were positively correlated with NO inhibition rate， and peaks 8， 10 （luteolin）， 13， 15， 3， 19， 17， 7 （ellagic acid）， 18， 1 were positively correlated with inhibition rate of TNF-α. The binding energies of vitexin， isovitexin and quercetin with iNOS protein receptor were less than －5.0 kcal/mol.

CONCLUSIONS

Vitexin， isovitexin and quercetin may be the basis of the anti-inflammatory effect of the petroleum ether extract from

M. dodecandrum

关键词

Keywords

references

雷后兴，李建良 . 中国畲药学 [M ] . 北京：人民军医出版社， 2014 ： 159 .

LEI H X ， LI J L . Chinese She pharmacy [M ] . Beijing ： People’s Military Medical Press ， 2014 ： 159 .

WANG J F ， JIA Z Y ， ZHANG Z H ， et al . Analysis of chemical constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-exactive focus-MS/MS [J ] . Molecules ， 2017 ， 22 （ 3 ）： 476 .

刘爽，毛佳乐，邱伟文，等 . 畲药地菍化学成分和药理作用研究进展 [J ] . 中国现代应用药学， 2024 ， 41 （ 7 ）： 996 - 1005 .

LIU S ， MAO J L ， QIU W W ， et al . Research progress on the chemical constituents and pharmacological effect of She medicine Melastoma dodecandrum Lour .[J ] . Chin J Mod Appl Pharm ， 2024 ， 41 （ 7 ）： 996 - 1005 .

刘静，明惠仪，麻秀萍，等 . 地菍中6种成分含量测定及其与抗氧化活性的相关性分析 [J ] . 中国药房， 2022 ， 33 （ 16 ）： 1962 - 1967 .

LIU J ， MING H Y ， MA X P ， et al . Content determination of 6 components in Melastoma dodecandrum and analysis of its correlation with antioxidant activity [J ] . China Pharm ， 2022 ， 33 （ 16 ）： 1962 - 1967 .

WENG J Y ， ZHOU J K ， LIANG L Q ， et al . UHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats [J ] . Pharm Biol ， 2019 ， 57 （ 1 ）： 807 - 815 .

张强，杨宇婷 . 中药谱效关系研究现状概述 [J ] . 时珍国医国药， 2022 ， 33 （ 3 ）： 680 - 683 .

ZHANG Q ， YANG Y T . Overview of the research status of the relationship between spectrum and effect of traditional Chinese medicine [J ] . Lishizhen Med Mater Med Res ， 2022 ， 33 （ 3 ）： 680 - 683 .

林婧，梁洁，黄春燕，等 . 壮药华佗豆不同极性部位HPLC指纹图谱的建立及其镇痛抗炎作用的谱效关系 [J ] . 中国药房， 2021 ， 32 （ 17 ）： 2079 - 2084 .

LIN J ， LIANG J ， HUANG C Y ， et al . Establishment of HPLC fingerprint of different polar parts of Zhuang medicine Calonyction muricatum and spectrum-effect relationship of its analgesic and anti-inflammatory effects [J ] . China Pharm ， 2021 ， 32 （ 17 ）： 2079 - 2084 .

侯小涛，陈晓璐，郝二伟，等 . 基于谱效关系的肉桂改善肾阳虚作用的质量标志物（Q-Marker）研究 [J ] . 中草药， 2021 ， 52 （ 9 ）： 2597 - 2607 .

HOU X T ， CHEN X L ， HAO E W ， et al . Quality marker study of Cinnamomi Cortex on improving kidney-Yang deficiency based on spectrum-effect relationship [J ] . Chin Tradit Herb Drugs ， 2021 ， 52 （ 9 ）： 2597 - 2607 .

邵冰梅，李小锋，闫蓓蓓，等 . 裸花紫珠醇提物UPLC指纹图谱建立及抗呼吸道合胞病毒谱效关系研究 [J ] . 中国药房， 2023 ， 34 （ 20 ）： 2465 - 2470 .

SHAO B M ， LI X F ， YAN B B ， et al . Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from Callicarpa nudiflora [J ] . China Pharm ， 2023 ， 34 （ 20 ）： 2465 - 2470 .

许晴，李智，万梅绪，等 . 中药活性成分筛选新技术研究进展 [J ] . 药物评价研究， 2021 ， 44 （ 7 ）： 1541 - 1547 .

XU Q ， LI Z ， WAN M X ， et al . Research progress on screening technology of active ingredients in traditional Chinese medicine [J ] . Drug Eval Res ， 2021 ， 44 （ 7 ）： 1541 - 1547 .

CHANG Y Q ， ZHANG D ， YANG G Y ， et al . Screening of anti-lipase components of Artemisia argyi leaves based on spectrum-effect relationships and HPLC-MS/MS [J ] . Front Pharmacol ， 2021 ， 12 ： 675396 .

KIM J G ， KIM M J ， LEE J S ， et al . Smilax guianensis Vitman extract prevents LPS-induced inflammation by inhibi-ting the NF-κB pathway in RAW 264.7 cells [J ] . J Microbiol Biotechnol ， 2020 ， 30 （ 6 ）： 822 - 829 .

Views

184

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Establishment of HPLC fingerprint of Shuangdong capsules and the spectral effect relationship of its anti-inflammatory effect

Study on the fingerprint of total saponins from Mussaenda pubescens and the spectrum-effect relationship of its hepatoprotective activity

Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from Callicarpa nudiflora

Fingerprint establishment of Huangqin decoction and study on spectrum-effect relationship of its antidermatophytic activity in different phase states

Screening of active ingredients for estrogenic effect of total flavonoids from Cuscutae Semen

Related Author

LI Li

LIU Xingcun

WU Jun

TANG Lei

HUANG Jiayu

YAO Qingqing

ZHANG Michan

LI Jia

Related Institution

School of Pharmaceutical Sciences， Guizhou Medical University

Long-Range Pharmaceuticals LTD

Guangxi Institute of Chinese Medicine & Pharmaceutical Science

Guangxi Key Laboratory of the Quality Standard of Traditional Chinese Medicine

Qingdao Academy of Chinese Medicinal Sciences， Shandong University of Traditional Chinese Medicine

⁰

Fundamental study on the anti-inflammatory activity of the petroleum ether extract from Melastoma dodecandrum based on spectrum-effect relationship

DOI：10.6039/j.issn.1001-0408.2024.23.07

摘要

Abstract

关键词

Keywords

references