Study on the differential markers of <italic style="font-style: italic">Morus alba </italic>leaves before and after baked with honey based on fingerprint and chemometrics

YAN Beibei; ZHANG Xiaoping; QIN Huanyun; ZHAO Xiangjun; SHAO Bingmei; TIAN Jingzhen; MA Dalong

doi:10.6039/j.issn.1001-0408.2024.10.08

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Study on the differential markers of Morus alba leaves before and after baked with honey based on fingerprint and chemometrics

更新时间：2025-09-25

- Study on the differential markers of Morus alba leaves before and after baked with honey based on fingerprint and chemometrics
- ZHONGGUO YAOFANG Vol. 35, Issue 10, Pages: 1198-1202(2024)
- 作者机构：
  
  1.山东中医药大学药学院，济南　250355
  2.山东中医药大学青岛中医药科学院，山东青岛　266000
  3.青岛大学附属医院药品调剂科，山东青岛　266005
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.10.08
  CLC： R284;R917
- Received：02 November 2023，
  
  Revised：2024-03-19，
  
  Accepted：22 March 2024，
  
  Published：30 May 2024
- 稿件说明：
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闫蓓蓓,张晓平,秦焕云,等.基于指纹图谱与化学计量学的桑叶蜜炙前后差异性标志物研究[J].中国药房,2024,35(10):1198-1202.

YAN Beibei,ZHANG Xiaoping,QIN Huanyun,et al.Study on the differential markers of Morus alba leaves before and after baked with honey based on fingerprint and chemometrics[J].ZHONGGUO YAOFANG,2024,35(10):1198-1202.
闫蓓蓓,张晓平,秦焕云,等.基于指纹图谱与化学计量学的桑叶蜜炙前后差异性标志物研究[J].中国药房,2024,35(10):1198-1202. DOI： 10.6039/j.issn.1001-0408.2024.10.08.

YAN Beibei,ZHANG Xiaoping,QIN Huanyun,et al.Study on the differential markers of Morus alba leaves before and after baked with honey based on fingerprint and chemometrics[J].ZHONGGUO YAOFANG,2024,35(10):1198-1202. DOI： 10.6039/j.issn.1001-0408.2024.10.08.

摘要

目的

比较桑叶蜜炙前后化学成分的变化，筛选差异性标志物并进行含量测定，为蜜炙桑叶的质量控制提供参考。

方法

采用高效液相色谱（HPLC）法建立桑叶蜜炙前后指纹图谱，标定共有峰并进行相似度评价；以生桑叶、蜜炙桑叶共有峰峰面积为指标，运用主成分分析和正交偏最小二乘-判别分析筛选桑叶蜜炙前后差异性标志物，并进行定量分析。

结果

从10批生桑叶、10批蜜炙桑叶HPLC指纹图谱中分别标定出23、24个共有峰，2类样品图谱的相似度均大于0.97；主成分分析结果显示，生桑叶和蜜炙桑叶分为2类，正交偏最小二乘-判别分析结果显示，峰2、峰H（5-羟甲基糠醛）、峰1、峰17（异绿原酸C）、峰16的变量重要性投影值均大于1；生桑叶、蜜炙桑叶中差异性标志物异绿原酸C的平均含量分别为0.093 6、0.127 8 mg/g，差异有统计学意义（

＜0.05）。

结论

筛选得到异绿原酸C等5种差异性标志物；桑叶蜜炙后异绿原酸C含量显著升高。

Abstract

OBJECTIVE

To compare the changes of chemical components of

Morus alba

leaves， screen differential markers， and determine their contents， so as to provide reference for quality control of

M. alba

leaves before and after baked with honey.

METHODS

The fingerprints of

M. alba

leaves before and after baked with honey were established by high-performance liquid chromatography （HPLC）. The common peaks of the fingerprints were identified and the similarity was evaluated. The differential markers of

M. alba

leaves before and after baked with honey were screened by principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） using common peak are of raw material and product baked with honey of

M. alba

leaves as index. The quantitative analysis was carried out.

RESULTS

Twenty-three and twenty-four common peaks were identified from the HPLC fingerprint spectra of ten batches of raw material and ten batches of product baked with honey of

M. alba

leaves， respectively. The similarities of HPLC fingerprints for raw material and product baked with honey of

M. alba

leaves were all greater than 0.97. The results of PCA showed that raw material and product baked with honey of

M. alba

leaves could be divided into two categories. The results of OPLS-DA showed that the variable importance in projection of peak 2， peak H （5-hydroxymethylfurfural）， peak 1， peak 17 （isochlorogenic acid C） and peak 16 were all greater than 1. The average contents of differential marker of isochlorogenic acid C in raw material and product baked with honey of

M. alba

leaves were 0.093 6 and 0.127 8 mg/g， respectively； there was statistical significance （

＜0.05）.

CONCLUSIONS

Five differential markers such as isochlorogenic acid C are obtained. The content of isochlorogenic acid C in

M. alba

leaves is significantly increased after baked with honey.

关键词

Keywords

references

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Study on the differential markers of Morus alba leaves before and after baked with honey based on fingerprint and chemometrics

DOI：10.6039/j.issn.1001-0408.2024.10.08

摘要

Abstract

关键词

Keywords

references