Effect and mechanism of <italic style="font-style: italic">Panax notoginseng </italic>saponins on wound healing after anal fistula surgery in rats

WANG Xinmin; LIU Yakun; LI Gang; LIU Juan; XU Huizhi; ZHANG Jingjie; LI Minlu; NIU Jingya; ZHANG Binggui

doi:10.6039/j.issn.1001-0408.2024.06.10

您当前的位置：

首页 >

文章列表页 >

Effect and mechanism of Panax notoginseng saponins on wound healing after anal fistula surgery in rats

更新时间：2025-09-25

- Effect and mechanism of Panax notoginseng saponins on wound healing after anal fistula surgery in rats
- ZHONGGUO YAOFANG Vol. 35, Issue 6, Pages: 695-700(2024)
- 作者机构：
  
  1.河北中医药大学研究生学院，石家庄　050200
  2.河北省第八人民医院普外肛肠科，石家庄　050024
  3.石家庄市中医院肛肠科，石家庄　050011
  4.石家庄市藁城人民医院普外肝胆科，石家庄　052160
  5.河北中医药大学中西医结合临床学院，石家庄　050200
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.06.10
  CLC： R285.5;R965
- Received：31 August 2023，
  
  Revised：2024-01-23，
  
  Accepted：05 February 2024，
  
  Published：30 March 2024
- 稿件说明：
移动端阅览
王鑫民,刘亚坤,李刚,等.三七总皂苷对肛瘘术后大鼠创面愈合的影响及机制[J].中国药房,2024,35(06):695-700.

WANG Xinmin,LIU Yakun,LI Gang,et al.Effect and mechanism of Panax notoginseng saponins on wound healing after anal fistula surgery in rats[J].ZHONGGUO YAOFANG,2024,35(06):695-700.
王鑫民,刘亚坤,李刚,等.三七总皂苷对肛瘘术后大鼠创面愈合的影响及机制[J].中国药房,2024,35(06):695-700. DOI： 10.6039/j.issn.1001-0408.2024.06.10.

WANG Xinmin,LIU Yakun,LI Gang,et al.Effect and mechanism of Panax notoginseng saponins on wound healing after anal fistula surgery in rats[J].ZHONGGUO YAOFANG,2024,35(06):695-700. DOI： 10.6039/j.issn.1001-0408.2024.06.10.

摘要

目的

探究三七总皂苷（PNS）调节缺氧诱导因子1α（HIF-1α）/血管内皮生长因子（VEGF）/血管内皮生长因子受体2（VEGFR2）信号通路对肛瘘术后大鼠创面愈合的影响及潜在机制。

方法

以SD大鼠为对象，采用大肠埃希菌液感染创面的方法复制肛瘘术后大鼠模型，并将其随机分为模型组，PNS低、高剂量组

（15、30 mg/cm

），PNS高剂量+2-甲氧基雌二醇（2ME2）组（PNS 30 mg/cm

+HIF-1α抑制剂2ME2 4 mg/kg），每组10只；另选10只正常大鼠，仅进行背部脱毛处理，作为对照组。各药物组大鼠肌内或（和）腹腔注射相应药液，每天1次，持续3周。末次给药后，检测各组大鼠的创面愈合率（对照组除外），创面组织微血管密度（MVD）和胶原蛋白Ⅰ（collagen Ⅰ）、纤维连接蛋白（FN）阳性表达情况，血清中血管生成相关因子[VEGF、血管生成素Ⅰ（Ang-Ⅰ）、Ang-Ⅱ

]

水平和血清、创面组织中炎症因子[白细胞介素6（IL-6）、IL-2

]

水平，以及创面组织中HIF-1α/VEGF/VEGFR2信号通路相关蛋白的表达情况。

结果

模型组大鼠创面组织MVD及collagen Ⅰ、FN表达水平，血清及创面组织中IL-6、IL-2水平均较对照组显著升高（

＜0.05），血清中VEGF、Ang-Ⅰ、Ang-Ⅱ水平均较对照组显著降低（

＜0.05）。PNS低、高剂量组大鼠创面愈合率，创面组织MVD，血清中VEGF、Ang-Ⅰ、Ang-Ⅱ水平，创面组织中collagen Ⅰ、FN表达水平和HIF-1α、VEGF、VEGFR2蛋白的表达水平均较模型组显著升高（

＜0.05），血清及创面组织中IL-6、IL-2水平均较模型组显著降低（

＜0.05），且高剂量PNS的作用更强（

＜0.05）；2ME2可减弱PNS对肛瘘术后大鼠上述指标的改善作用（

＜0.05）。

结论

PNS可促进血管生成相关因子产生并抑制促炎因子生成，从而促进肛瘘术后大鼠创面的愈合，上述作用与激活HIF-1α/VEGF/VEGFR2信号通路有关。

Abstract

OBJECTIVE

To investigate the effect and mechanism of

Panax notoginseng

saponins （PNS） on wound healing after anal fistula surgery in rats by regulating the hypoxia-inducible factor-1α （HIF-1α）/vascular endothelial growth factor （VEGF）/vascular endothelial growth factor receptor-2 （VEGFR2） signaling pathway.

METHODS

SD rats were selected to establish a postoperative rat model of anal fistula by infecting wound with

Escherichia coli

. The model rats were randomly grouped into model group， PNS low-dose and high-dose groups （15， 30 mg/cm

）， high-dose of PNS+2-methoxyestradiol （2ME2） group （PNS 30 mg/cm

+HIF-1α inhibitor 2ME2 4 mg/kg）， with 10 rats in each group. Another 10 normal rats were selected for back hair removal treatment as the control group. Each drug group was injected with the corresponding drug solution intramuscularly or （and） intraperitoneally， once a day， for 3 weeks. After the last administration， the wound healing rate （excl

uding the control group）， microvascular density （MVD）， the expression of collagen Ⅰ and fibronectin （FN） in the wound tissue were detected in each group； the levels of angiogenic factors [VEGF， angiopoietin-Ⅰ （Ang-Ⅰ）， Ang-Ⅱ

]

in serum， the levels of inflammatory factors [interleukin-6 （IL-6） and IL-2

]

in serum and wound tissue as well as the expressions of the related proteins of HIF-1α/VEGF/VEGFR2 signaling pathway in the wound tissue of rats were also detected in each group.

RESULTS

The MVD， the expression of collagen Ⅰ and FN in the wound tissue， and the levels of IL-6 and IL-2 in serum and wound tissue of rats increased significantly in the model group， compared to the control group （

＜0.05）， while the serum levels of VEGF， Ang-Ⅰ and Ang-Ⅱ decreased significantly （

＜0.05）. The wound healing rate， the MVD in wound tissue， the serum levels of VEGF， Ang-Ⅰ and Ang-Ⅱ， the expressions of collagen Ⅰ and FN in the wound tissue， and protein expressions of HIF-1α， VEGF and VEGFR2 in the PNS low-dose and high-dose groups increased significantly， compared to the model group （

＜0.05）， while the levels of IL-6 and IL-2 in serum and wound tissue decreased significantly （

＜0.05）； the high-dose PNS had a stronger effect （

＜0.05）. 2ME2 could weaken the effect of PNS on above indicators of rats after anal fistula surgery （

＜0.05）.

CONCLUSIONS

PNS can promote the production of angiogenic factors and inhibit the production of pro-inflammatory factors， thereby promoting wound healing in rats after anal fistula surgery. The above effects are related to the activation of HIF-1α/VEGF/VEGFR2 signaling pathway.

关键词

Keywords

references

SCHOUTEN W R ， ARKENBOSCH J H C ， VAN DER WOUDE C J ， et al . Efficacy and safety of autologous adipose-derived stromal vascular fraction enriched with platelet-rich plasma in flap repair of transsphincteric cryptoglandular fistulas [J ] . Tech Coloproctol ， 2021 ， 25 （ 12 ）： 1301 - 1309 .

PORWAL A ， KUNDU G C ， BHAGWAT G ， et al . Polyherbal formulation Anoac-H suppresses the expression of RANTES and VEGF for the management of bleeding hemorrhoids and fistula [J ] . Mol Med Rep ， 2021 ， 24 （ 4 ）： 736 .

ZHU Y ， WANG Y M ， JIA Y C ， et al . Roxadustat promotes angiogenesis through HIF-1α/VEGF/VEGFR2 signaling and accelerates cutaneous wound healing in diabetic rats [J ] . Wound Repair Regen ， 2019 ， 27 （ 4 ）： 324 - 334 .

YOUNIS N S ， MOHAMED M E ， EL SEMARY N A . Green synthesis of silver nanoparticles by the cyanobacteria Synechocystis sp.：characterization，antimicrobial and diabetic wound-healing actions [J ] . Mar Drugs ， 2022 ， 20 （ 1 ）： 56 .

MA X N ， ZHANG X ， KONG Y H ， et al . Therapeutic effects of Panax notoginseng saponins in rheumatoid arthritis：network pharmacology and experimental validation [J ] . Bioengineered ， 2022 ， 13 （ 6 ）： 14438 - 14449 .

DU Y Q ， CHEN W J ， LI Y S ， et al . Study on the regulatory effect of Panax notoginseng saponins combined with bone mesenchymal stem cell transplantation on IRAK1/TRAF6-NF-κB pathway in patients with diabetic cuta-neous ulcers [J ] . J Orthop Surg Res ， 2023 ， 18 （ 1 ）： 80 .

LI Q Q ， LIANG X Y ， YANG Y F ， et al . Panax notoginseng saponins ameliorate cisplatin-induced mitochondrial injury via the HIF-1α/mitochondria/ROS pathway [J ] . FEBS Open Bio ， 2020 ， 10 （ 1 ）： 118 - 126 .

王孝，丁博文，郑薇，等 . 扶正生肌油纱对大鼠肛瘘术后瘢痕抑制作用及对血清TGF-β 1 表达的影响 [J ] . 齐齐哈尔医学院学报， 2022 ， 43 （ 19 ）： 1806 - 1810 .

WANG X ， DING B W ， ZHENG W ， et al . Inhibitory effect of Fuzheng shengji oil gauze on postoperative scarring in rats with anal fistula and its effect on the expression of serum TGF-β 1 [J ] . J Qiqihar Med Univ ， 2022 ， 43 （ 19 ）： 1806 - 1810 .

QU Y ， ZHANG Z J ， LU Y F ， et al . RNA sequencing reveals the wound repair mechanism of cuyuxunxi prescription in surgical patients with anal fistulas [J ] . Comb Chem High Throughput Screen ， 2022 ， 25 （ 8 ）： 1284 - 1293 .

LITTA F ， PAPAIT A ， LUCCHETTI D ， et al . The pathogenesis of cryptoglandular anal fistula：new insight into the immunological profile [J ] . Colorectal Dis ， 2022 ， 24 （ 12 ）： 1567 - 1575 .

MEN S Y ， HUO Q L ， SHI L ， et al . Panax notoginseng saponins promotes cutaneous wound healing and suppresses scar formation in mice [J ] . J Cosmet Dermatol ， 2020 ， 19 （ 2 ）： 529 - 534 .

KANG Z N ， XIAO Q ， WANG L L ， et al . The combination of astragaloside Ⅳ and Panax notoginseng saponins attenuates cerebral ischaemia-reperfusion injury in rats through ferroptosis and inflammation inhibition via activating Nrf2 [J ] . J Pharm Pharmacol ， 2023 ， 75 （ 5 ）： 666 - 676 .

LEI T ， GAO Y ， DUAN Y H ， et al . Panax notoginseng saponins improves healing of high glucose-induced wound through the GSK-3β/β-catenin pathway [J ] . Environ Toxicol ， 2022 ， 37 （ 8 ）： 1867 - 1877 .

YANG J T ， TANG L H ， ZHANG F J ， et al . Sevoflurane preconditioning promotes mesenchymal stem cells to relieve myocardial ischemia/reperfusion injury via TRPC6-induced angiogenesis [J ] . Stem Cell Res Ther ， 2021 ， 12 （ 1 ）： 584 .

WANG Y Y ， ZHU J Y ， CHEN J ， et al . The signaling pathways induced by exosomes in promoting diabetic wound healing：a mini-review [J ] . Curr Issues Mol Biol ， 2022 ， 44 （ 10 ）： 4960 - 4976 .

Views

162

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Pharmacodynamic evaluation of Polygonum capitatum nanofiber functional dressing

Study on Repair ，Anti-inflammation and Analgesia Effects of Compound Crocodile Oil Burn Ointment on Super- ficial Second-degree Burned Skin

Related Author

LYU Yuanju

WU Wenli

LIU Zemei

ZHENG Guangyan

WANG Lihong

CHE Xin

PAN Xiang

HAN Sijie

Related Institution

School of Pharmacy， Guizhou University of Traditional Chinese Medicine

⁰

Effect and mechanism of Panax notoginseng saponins on wound healing after anal fistula surgery in rats

DOI：10.6039/j.issn.1001-0408.2024.06.10

摘要

Abstract

关键词

Keywords

references