Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema

LI Jingfeng; SHI Zhengyuan; ZHANG Mengjie; GUAN Lei; JIANG Dechun

doi:10.6039/j.issn.1001-0408.2024.04.18

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Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema

更新时间：2025-09-25

- Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema
- ZHONGGUO YAOFANG Vol. 35, Issue 4, Pages: 476-480(2024)
- 作者机构：
  
  1.首都医科大学附属北京世纪坛医院药剂科，北京　100038
  2.首都医科大学附属北京世纪坛医院麻醉科，北京　100038
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2024.04.18
  CLC： R969.3;R917
- Received：24 August 2023，
  
  Revised：2024-01-27，
  
  Accepted：31 January 2024，
  
  Published：29 February 2024
- 稿件说明：
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李京峰,时正媛,张梦洁,等.丙泊酚血药浓度检测方法建立及在淋巴水肿患者中的应用[J].中国药房,2024,35(04):476-480.

LI Jingfeng,SHI Zhengyuan,ZHANG Mengjie,et al.Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema[J].ZHONGGUO YAOFANG,2024,35(04):476-480.
李京峰,时正媛,张梦洁,等.丙泊酚血药浓度检测方法建立及在淋巴水肿患者中的应用[J].中国药房,2024,35(04):476-480. DOI： 10.6039/j.issn.1001-0408.2024.04.18.

LI Jingfeng,SHI Zhengyuan,ZHANG Mengjie,et al.Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema[J].ZHONGGUO YAOFANG,2024,35(04):476-480. DOI： 10.6039/j.issn.1001-0408.2024.04.18.

摘要

目的

建立测定人血浆中丙泊酚浓度的方法并应用于淋巴水肿患者血药浓度检测。

方法

以麝香草酚为内标，血浆样品经蛋白沉淀后，采用超高效液相色谱-串联质谱（UPLC-MS/MS）法测定丙泊酚的浓度。以Kinetex C

为色谱柱，以乙腈（A）-水（B）为流动相进行梯度洗脱，流速为200 μL/min，进样量为5 μL，柱温为40 ℃，样品仓温度为15 ℃。采用多反应监测模式进行检测，用于定量分析的离子对为

177.0→161.2（丙泊酚）、

149.0→133.1（内标）。采用上述方法测定6例淋巴水肿患者血浆中丙泊酚的血浆浓度。

结果

丙泊酚检测质量浓度的线性范围为50～5 000 ng/mL（

＝0.995 0）；批内、批间精密度的RSD均不高于8.08%；空白血浆中无内源性干扰；无残留效应和稀释效应；提取回收率为89.80%～93.73%，基质效应为9

7.93%～101.73%；稳定性试验的RSD均小于3.27%。术中靶控输注2～30 min内，6例患者的丙泊酚血药浓度为1 865.3～6 056.2 ng/mL，停药后4～8 h内基本代谢完全。

结论

所建UPLC-MS/MS法可以在不进行衍生化的前提下，实现对丙泊酚的测定，样品前处理过程简便、快速，可用于淋巴水肿患者血浆样本中丙泊酚的血药浓度检测。

Abstract

OBJECTIVE

To establish a method for the determination of propofol concentration in human plasma and apply it in patients with lymphedema.

METHODS

The concentration of propofol was determined by UPLC-MS/MS after protein precipitation of plasma samples using thymol as internal standard. The sample was eluted on a Kinetex C

column with a mobile phase consisting of acetonitrile （A）-water （B） for gradient elution at the flow rate of 200 μL/min. The sample size was 5 μL， and the column temperature was set at 40 ℃. The sample chamber temperature was 15 ℃. Using multi-reaction monitoring mode， the ion pairs for quantitative analysis were

m/z

177.0→161.2 （propofol） and

m/z

149.0→133.1 （internal standard）， respectively. The above method was used to determine the plasma concentration of propofol in 6 patients with lymphedema.

RESULTS

The linear range of propofol was 50-5 000 ng/mL （

＝0.995 0）. RSDs of within- and between-batch precision were not more than 8.08%； no endogenous interference， carryover effect， or dilution effect was observed in blank plasma. The extraction recovery ranged from 89.80% to 93.73%， and matrix effects were within the range of 97.93%-101.73%. RSDs of the stability test were all lower than 3.27%. During intraoperative TCI 2-30 min， the plasma concentration of propofol in 6 patients was maintained in the range of 1 865.3-6 056.2 ng/mL， and the propofol was almost excreted within 4-8 h after operation.

CONCLUSIONS

The established UPLC-MS/MS method in this study can achieve the determination of propofol and a simple and fast sample pretreatment process without derivatization； it is proved to be suitable for the concentration monitoring of propofol in plasma samples of patients with lymphedema.

关键词

Keywords

references

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